Document Detail


Design and synthesis of novel indole beta-diketo acid derivatives as HIV-1 integrase inhibitors.
MedLine Citation:
PMID:  15456274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Diketo acids such as S-1360 (1A) and L-731,988 (2) are potent and selective inhibitors of HIV-1 integrase (IN). A plethora of diketo acid-containing compounds have been claimed in patent literature without disclosing much biological activities and synthetic details (reviewed in Neamati, N. Exp. Opin. Ther. Pat. 2002, 12, 709-724). To establish a coherent structure-activity relationship among the substituted indole nucleus bearing a beta-diketo acid moiety, a series of substituted indole-beta-diketo acids (4a-f and 5a-e) were synthesized. All compounds tested showed anti-IN activity at low micromolar concentrations with varied selectivity against the strand transfer process. Three compounds, the indole-3-beta-diketo acids 5a and 5c, and the parent ester 9c, have shown an antiviral activity in cell-based assays. We further confirmed a keto-enolic structure in the 2,3-position of the diketo acid moiety of a representative compound (4c) using NMR and X-ray crystallographic analysis. Using this structure as a lead for all of our computational studies, we found that the title compounds extensively interact with the essential amino acids on the active site of IN.
Authors:
Mario Sechi; Massimiliano Derudas; Roberto Dallocchio; Alessandro Dessì; Alessia Bacchi; Luciano Sannia; Fabrizio Carta; Michele Palomba; Omar Ragab; Carney Chan; Robert Shoemaker; Shizuko Sei; Raveendra Dayam; Nouri Neamati
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medicinal chemistry     Volume:  47     ISSN:  0022-2623     ISO Abbreviation:  J. Med. Chem.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-30     Completed Date:  2004-11-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9716531     Medline TA:  J Med Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5298-310     Citation Subset:  IM    
Affiliation:
Dipartimento Farmaco Chimico Tossicologico, Università di Sassari, Via Muroni 23/A, 07100 Sassari, Italy. mario.sechi@uniss.it
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Crystallography, X-Ray
Drug Design
HIV Integrase / chemistry,  metabolism*
HIV Integrase Inhibitors / chemical synthesis*,  chemistry,  pharmacology
HIV-1 / drug effects*
Humans
Indoles / chemical synthesis*,  chemistry,  pharmacology
Keto Acids / chemical synthesis*,  chemistry,  pharmacology
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Structure
Solutions
Structure-Activity Relationship
T-Lymphocytes / virology
Grant Support
ID/Acronym/Agency:
N01 CO 12400/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/HIV Integrase Inhibitors; 0/Indoles; 0/Keto Acids; 0/Solutions; EC 2.7.7.-/HIV Integrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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