| Design, synthesis and biological application of chemical probes for bio-imaging. | |
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MedLine Citation:
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PMID: 20372693 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Biological imaging has revealed many new biological findings, among them GFP and other fluorescent proteins and small molecule based fluorescent probes have been widely used, especially in the past two decades. In this tutorial review the design concept and application of chemical probes are described-these are FRET based probes, Zn(2+) probes and MRI probes. Fluorescence resonance energy transfer (FRET) has been used extensively for the design principle for fluorescent probes. One of the most significant advantages of probes with FRET modulation is that these can enable ratiometric measurement in living cells, which reduces the artefacts from microscopic imaging systems. The design strategy for the development of a small molecular FRET probe is described, in terms of avoiding close contact between donor fluorophore and acceptor fluorophore in aqueous solution. Furthermore, a strategy to design a FRET probe with modulating overlap integral of donor and acceptor is introduced. Numerous tools for Zn(2+) sensing in living cells have become available in the last decade. Among them, fluorescence imaging using fluorescent probes has been the most popular approach. Some of these probes can be used to visualize Zn(2+) in living cells. Some of the biological functions of Zn(2+) were clarified using these probes, especially in neuronal cells, which contain a high concentration of free Zn(2+). Real-time imaging of enzyme activities in vivo offers valuable information in understanding living systems and in developing medicine for various types of diseases. Magnetic resonance imaging (MRI) is expected to be one of the most promising in vivo imaging techniques in the scientific and medical fields. One of the most promising nuclide for MRI is (19)F. (19)F atoms are concentrated in the form of solid salts mostly in bones and teeth in our bodies. The MRI signal of the intrinsic (19)F is hardly detectable. A novel design strategy for (19)F MRI probes in detecting protease activity is described. |
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Authors:
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Kazuya Kikuchi |
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Publication Detail:
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Type: Journal Article; Review Date: 2010-04-06 |
Journal Detail:
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Title: Chemical Society reviews Volume: 39 ISSN: 1460-4744 ISO Abbreviation: Chem Soc Rev Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-08-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0335405 Medline TA: Chem Soc Rev Country: England |
Other Details:
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Languages: eng Pagination: 2048-53 Citation Subset: IM |
Affiliation:
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Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita City, Osaka 565-0871, Japan. kkikuchi@mls.eng.osaka-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescence Resonance Energy Transfer Fluorescent Dyes / chemical synthesis*, chemistry Green Fluorescent Proteins / chemistry Magnetic Resonance Imaging / methods* Zinc / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 147336-22-9/Green Fluorescent Proteins; 7440-66-6/Zinc |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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