Document Detail


Design and operation of a dual laser chromosome sorter.
MedLine Citation:
PMID:  6184209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe the design and operation of a dual laser fluorescence activated chromosome sorter that can analyze and sort chromosome suspensions stained with two complementary DNA stains. We used two 18-watt argon-ion lasers to provide maximal excitation of the DNA-specific stains Hoechst 33258 and chromomycin A3. The lasers are focused onto the sample stream by independent optics permitting optimal focusing of any pair of laser wavelengths. We incorporated beam expanders to decrease the energy density on the optics and improve the focal spot uniformity. Adjustable focusing lens holders are used to set the optimal focal spot size. The redesigned sorter separates suspensions of normal human fibroblast chromosomes into 21 fractions and can reliably sort more pure chromosome fractions than was previously possible.
Authors:
R V Lebo; A M Bastian
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytometry     Volume:  3     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-02-14     Completed Date:  1983-02-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  213-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bisbenzimidazole
Cell Line
Cell Separation / instrumentation*
Chromomycin A3
Chromosomes, Human* / analysis
DNA / analysis
Flow Cytometry / instrumentation*
Humans
Lasers
Staining and Labeling
Chemical
Reg. No./Substance:
23491-44-3/Bisbenzimidazole; 7059-24-7/Chromomycin A3; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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