Document Detail


Design of nevirapine derivatives insensitive to the K103N and Y181C HIV-1 reverse transcriptase mutants.
MedLine Citation:
PMID:  16644557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nevirapine (Viramune) belongs to the first generation of non-nucleoside reverse transcriptase inhibitors (NNRTIs). Its efficiency is limited by drug resistant mutations, such as K103N and Y181C, so, the aim of this work was to design novel nevirapine analogues insensitive to the K103N and Y181C HIV-1 RT. 360 Nevirapine derivatives were designed using a combinatorial library design approach and these compounds were docked into the binding pocket of mutant HIV-1 RT enzyme structures, using the GOLD program. 124 Compounds having a GoldScore higher than that of nevirapine (55.00 and 52.00 for K103N and Y181C mutants, respectively) were first retrieved and submitted to a topological analysis with the SILVER program. Consequently, 31 compounds presenting a significant percentage of the surfaces buried upon binding (>80%) and exhibiting hydrogen bonds to either N103 or C181 residues of the HIV-RT were selected. To ensure that these compounds had hydrogen bonding interaction to either N103 or C181 residues, their interaction energies were estimated by quantum chemical calculations (QCCs). Finally, QCCs represent an alternative method for performing post docking procedure.
Authors:
P Saparpakorn; S Hannongbua; D Rognan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  SAR and QSAR in environmental research     Volume:  17     ISSN:  1062-936X     ISO Abbreviation:  SAR QSAR Environ Res     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-28     Completed Date:  2006-07-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9440156     Medline TA:  SAR QSAR Environ Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  183-94     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Anti-HIV Agents / chemistry*
Combinatorial Chemistry Techniques
Drug Design
Drug Resistance
HIV Reverse Transcriptase / genetics*
Mutation*
Nevirapine / analogs & derivatives,  chemistry*
Reverse Transcriptase Inhibitors / chemistry*
Chemical
Reg. No./Substance:
0/Anti-HIV Agents; 0/Reverse Transcriptase Inhibitors; 129618-40-2/Nevirapine; EC 2.7.7.49/HIV Reverse Transcriptase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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