Document Detail


Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae.
MedLine Citation:
PMID:  23339912     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.
METHODS: Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.
RESULTS: According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.
CONCLUSIONS: Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.
Authors:
Fooladi Aa Imani; Islamieh D Iman; Doust R Hosseini; A Karami; Sm Marashi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Asian Pacific journal of tropical medicine     Volume:  6     ISSN:  1995-7645     ISO Abbreviation:  Asian Pac J Trop Med     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101533720     Medline TA:  Asian Pac J Trop Med     Country:  Singapore    
Other Details:
Languages:  eng     Pagination:  115-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved.
Affiliation:
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: imanifouladi.a@bmsu.ac.ir.
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