| Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae. | |
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MedLine Citation:
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PMID: 23339912 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously. METHODS: Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method. RESULTS: According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI. CONCLUSIONS: Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran. |
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Authors:
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Fooladi Aa Imani; Islamieh D Iman; Doust R Hosseini; A Karami; Sm Marashi |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Asian Pacific journal of tropical medicine Volume: 6 ISSN: 1995-7645 ISO Abbreviation: Asian Pac J Trop Med Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101533720 Medline TA: Asian Pac J Trop Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 115-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: imanifouladi.a@bmsu.ac.ir. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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