Document Detail


Design of a highly potent anti-hypertensive peptide based on ovokinin(2-7).
MedLine Citation:
PMID:  12162540     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ovokinin(2-7) (RADHPF), an orally active antihypertensive peptide derived from ovalbumin, lowers blood pressure in SHRs at a dose of 10 mg/kg. Attempts were made to potentiate its anti-hypertensive activity by replacing the amino acid residues in [Pro2, Phe3]-ovokinin(2-7), which was previously reported to have 33-fold stronger activity than ovokinin(2-7). The anti-hypertensive activity of [Pro2, Phe3]-ovokinin(2-7) was improved by replacement of the C-terminal Phe residue with Trp. Then, the best amino acid residues at other positions for the anti-hypertensive effect were selected. RPLKPW, the most potent derivative obtained, showed significant anti-hypertensive activities at a dose of 0.1 mg/kg after oral administration in spontaneously hypertensive rats (SHRs). Thus, RPLKPW showed 100-fold more potent anti-hypertensive activity than ovokinin(2-7).
Authors:
Yuko Yamada; Nobuyuki Matoba; Hachiro Usui; Kunihiko Onishi; Masaaki Yoshikawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  66     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-08-06     Completed Date:  2003-03-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1213-7     Citation Subset:  IM    
Affiliation:
Division of Food Bioscience and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Japan.
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Amino Acid Sequence
Animals
Antihypertensive Agents / administration & dosage,  chemistry*,  metabolism,  pharmacology*
Blood Pressure / drug effects
Dose-Response Relationship, Drug
Drug Design*
Injections, Intravenous
Male
Ovalbumin / chemistry*
Pancreatin / metabolism
Peptide Fragments / chemistry*
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Time Factors
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Peptide Fragments; 0/ovalbumin (359-364); 8049-47-6/Pancreatin; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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