| Design and evaluation of a novel nanoparticulate-based formulation encapsulating a HIP complex of lysozyme. | |
| | |
MedLine Citation:
|
PMID: 23137392 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Formulation development of protein therapeutics using polymeric nanoparticles has found very little success in recent years. Major formulation challenges include rapid denaturation, susceptibility to lose bioactivity in presence of organic solvents and poor encapsulation in polymeric matrix. In the present study, we have prepared hydrophobic ion pairing (HIP) complex of lysozyme, a model protein, using dextran sulfate (DS) as a complexing polymer. We have optimized the process of formation and dissociation of HIP complex between lysozyme and DS. The effect of HIP complexation on enzymatic activity of lysozyme was also studied. Nanoparticles were prepared and characterized using spontaneous emulsion solvent diffusion method. Furthermore, we have also investigated release of lysozyme from nanoparticles along with its enzymatic activity. Results of this study indicate that nanoparticles can sustain the release of lysozyme without compromising its enzymatic activity. HIP complexation using a polymer may also be employed to formulate sustained release dosage forms of other macromolecules with enhanced encapsulation efficiency. |
| | |
Authors:
|
Ripal Gaudana; Mitan Gokulgandhi; Varun Khurana; Deep Kwatra; Ashim K Mitra |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
|
Title: Pharmaceutical development and technology Volume: - ISSN: 1097-9867 ISO Abbreviation: Pharm Dev Technol Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9610932 Medline TA: Pharm Dev Technol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Par Pharmaceutical Companies, Inc , One Ram Ridge Road, Spring Valley, NY , USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in sil...
Next Document: Skeletal myogenic differentiation of urine-derived stem cells and angiogenesis using microbeads load...