| Design and construction of a compact end-station at NSRRC for circular-dichroism spectra in the vacuum-ultraviolet region. | |
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MedLine Citation:
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PMID: 20975221 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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A synchrotron-radiation-based circular-dichroism end-station has been implemented at beamline BL04B at the National Synchrotron Radiation Research Center (NSRRC) in Taiwan for biological research. The design and performance of this compact end-station for measuring circular-dichroism spectra in the vacuum-ultraviolet region are described. The linearly polarized light from the beamline is converted to modulated circularly polarized light with a LiF photoelastic modulator to provide a usable wavelength region of 130-330 nm. The light spot at the sample position is 5 mm × 5 mm at a slit width of 300 µm and provides a flux greater than 1 × 10(11) photons s(-1) (0.1% bandwidth)(-1). A vacuum-compatible cell made of two CaF(2) windows has a variable path length from 1.3 µm to 1 mm and a temperature range of 253-363 K. Measured CD spectra of (1S)-(+)-10-camphorsulfonic acid and proteins demonstrated the ability of this system to extend the wavelength down to 172 nm in aqueous solution and 153 nm in hexafluoro-2-propanol. |
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Authors:
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Szu Heng Liu; Yi Hung Lin; Liang Jen Huang; Shiang Wen Luo; Wan Lin Tsai; Su Yu Chiang; Hok Sum Fung |
Publication Detail:
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Type: Journal Article Date: 2010-10-01 |
Journal Detail:
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Title: Journal of synchrotron radiation Volume: 17 ISSN: 1600-5775 ISO Abbreviation: J Synchrotron Radiat Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9888878 Medline TA: J Synchrotron Radiat Country: Denmark |
Other Details:
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Languages: eng Pagination: 761-8 Citation Subset: IM |
Affiliation:
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National Synchrotron Radiation Research Center, 101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu 30076, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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