Document Detail


Design of carbohydrate multiarrays.
MedLine Citation:
PMID:  16466673     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, microarray technology has increasingly been widely applied in glycobiology. This technology has rather evident potential advantages: unlimited number of carbohydrate ligands coated onto one small sized chip, enormously low consumption of both carbohydrate ligands and carbohydrate-binding proteins to be tested, etc. Literature data demonstrate that three approaches are used for glycoarray design. The first one is based on the physical adsorption of glycomolecules on a surface (as in a common ELISA), the second one-on covalent immobilization, and the third one-on a streptavidin-biotin system. In all of the described methods, carbohydrate ligands were placed on chips as a 2D monolayer and high sensitivity was achieved due to fluorescent detection. Notably, a tendency of stepping from model chips toward real multiarrays, where the number of carbohydrate ligands can be up to two hundred, has been observed the last 2 years, this already producing a number of interesting findings when studying carbohydrate-binding proteins. In 2005 new construction, 3D glycochip was described, where 150 mum diameter polyacrylamide gel elements serve as microreactors instead of 2D dots. As a result of the 3D placement of a ligand, two orders of magnitude increase of its density is possible, this providing principal signal improvement during fluorescent detection and increasing method sensitivity. At the same time, carbohydrate consumption is low, i.e., approximately 1 pmol per gel element. Copolymerization chemistry enables the immobilization of several glycomolecule classes to the gel, in particular, aminospacered oligosaccharides, polyacrylamide conjugates, and even 2-aminopyridine derivatives of oligosaccharides, which are widely used in the structural analysis of glycoprotein N-chains.
Authors:
V I Dyukova; N V Shilova; O E Galanina; A Yu Rubina; N V Bovin
Related Documents :
17594493 - Estimation and correction of non-specific binding in a large-scale spike-in experiment.
19822003 - Base--2nd generation software for microarray data management and analysis.
11641033 - Comparison of the prediction of extremely low birth weight neonatal mortality by regres...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2006-01-05
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1760     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-25     Completed Date:  2006-06-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  603-9     Citation Subset:  IM    
Affiliation:
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Carbohydrates / chemistry*,  diagnostic use*
Gels
Ligands
Microarray Analysis / methods*
Polymers
Proteins / chemistry
Grant Support
ID/Acronym/Agency:
GM60938/GM/NIGMS NIH HHS; GM62116/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Gels; 0/Ligands; 0/Polymers; 0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Thermodynamic properties of enzyme-catalyzed reactions involving guanine, xanthine, and their nucleo...
Next Document:  Value of fat-suppressed PD-weighted TSE-sequences for detection of anterior and posterior cruciate l...