| The Design, Selection, and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs. | |
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MedLine Citation:
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PMID: 23027048 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The efficient and specific silencing of genes via RNA interference (RNAi) for functional genomics and therapeutics depends on careful consideration of the factors that affect the functionality of small interfering RNA (siRNA). These factors include (1) the length of sequence available for siRNA targeting of an mRNA, (2) the structural and thermodynamic properties of target and siRNA sequences, (3) the mechanisms of siRNA off-target effects, and (4) the susceptibility of siRNA degradation when exposed to nucleases in serum and inside cells. Incorporation of Unlocked Nucleobase analogs (UNAs) in the siRNA design offers an attractive approach to design highly efficacious siRNAs with dramatically reduced off-target activity. Here, we describe methods and principles pertaining to the design, selection and screening of optimal siRNAs containing UNA. |
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Authors:
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Narendra Vaish; Pinky Agarwal |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 942 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 111-34 Citation Subset: IM |
Affiliation:
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Vaish Biotech Consulting, Kirkland, WA, USA, vaishn1@gmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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