Document Detail


Design and Screening of siRNAs Against Highly Structured RNA Targets.
MedLine Citation:
PMID:  23027046     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
RNA silencing is an invaluable tool to interrogate gene function. The cytoplasmic delivery of small interfering RNAs (siRNAs) complementary to a gene of interest results in cleavage and degradation of the target mRNA. Given the potential to target virtually any RNA, siRNA-based therapeutics may revolutionize the treatment of disease. Target site accessibility is a significant barrier to the design and efficacy of siRNAs, particularly against highly structured targets such as the genomes of positive-sense RNA viruses. Here, we describe a bead-based approach to screen for target site accessibility of siRNAs designed against highly structured target RNAs and demonstrate that this approach can be used to assess target site accessibility in vitro and predict potent target sites for siRNAs in cell culture against a highly structured RNA target.
Authors:
Neda Nasheri; John Paul Pezacki; Selena M Sagan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  942     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  69-86     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Microbiology, & Immunology, Steacie Institute for Molecular Sciences, National Research Council of Canada, and Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON, Canada.
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