Document Detail

Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors.
MedLine Citation:
PMID:  23027059     Owner:  NLM     Status:  In-Data-Review    
RNAi has become a very versatile tool to silence gene expression in a variety of organisms, in particular when classical genetic methods are missing. However, the application of this method in functional studies has raised new challenges in order to design RNAi reagents that minimize false positives and false negatives. Because the performance of reagents cannot be validated on a genome-wide scale, improved computational methods are required that consider experimentally derived quality measures. In this chapter, we describe computational methods for the design of RNAi reagents for invertebrate model organisms and human disease vectors, such as Anopheles. We describe procedures for designing short and long double-stranded RNAs for single genes, and evaluate their predicted specificity and efficiency. Using a bioinformatics pipeline we also describe how to design a genome-wide RNAi library for Anopheles gambiae.
Thomas Horn; Michael Boutros
Related Documents :
8343889 - Choosing a powerful lithotriptor.
15861819 - A tunable multivariable nonlinear robust observer for biological systems.
15926949 - The self, the symbolic and synchronicity: virtual realities and the emergence of the ps...
23642009 - What google maps can do for biomedical data dissemination: examples and a design study.
24311059 - Algorithmic complexity for short binary strings applied to psychology: a primer.
24040139 - The case for using the repeatability coefficient when calculating test-retest reliability.
16719619 - Comparing patients' predicted test scores from a regression equation with their obtaine...
9110039 - Quasi-induced exposure: methodology and insight.
18593489 - Anatomic dead space cannot be predicted by body weight.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  942     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  315-46     Citation Subset:  IM    
Department of Cell and Molecular Biology, Heidelberg University, Heidelberg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Production and Application of Long dsRNA in Mammalian Cells.
Next Document:  Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst ...