| Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA). | |
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MedLine Citation:
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PMID: 23027050 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Small interfering RNAs (siRNAs) are potent inducers of RNA interference-the conservative cellular process of posttranscriptional gene silencing. The silencing activity of siRNAs depends on the thermodynamic asymmetry of the siRNA duplex. Here, we describe the design of chemically modified fork-like siRNA (fsiRNAs) containing mismatches at the 3'-end region of the sense strand and 2'-O-methyl modifications in nuclease-sensitive sites, capable of silencing of thermodynamically unfavorable targets. |
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Authors:
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Elena L Chernolovskaya; Marina A Zenkova |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 942 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 153-68 Citation Subset: IM |
Affiliation:
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Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia, elena_ch@niboch.nsc.ru. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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