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Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA).
MedLine Citation:
PMID:  23027050     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Small interfering RNAs (siRNAs) are potent inducers of RNA interference-the conservative cellular process of posttranscriptional gene silencing. The silencing activity of siRNAs depends on the thermodynamic asymmetry of the siRNA duplex. Here, we describe the design of chemically modified fork-like siRNA (fsiRNAs) containing mismatches at the 3'-end region of the sense strand and 2'-O-methyl modifications in nuclease-sensitive sites, capable of silencing of thermodynamically unfavorable targets.
Authors:
Elena L Chernolovskaya; Marina A Zenkova
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  942     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-68     Citation Subset:  IM    
Affiliation:
Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia, elena_ch@niboch.nsc.ru.
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