Document Detail

Design and Implementation of Two-Dimensional Polymer Adsorption Models: Evaluating the Stability of Candida antarctica Lipase B/Solid-Support Interfaces by QCM-D.
MedLine Citation:
PMID:  23286367     Owner:  NLM     Status:  Publisher    
A two-dimensional model of a solid-supported enzyme catalyst bead is fabricated on a quartz crystal microbalance with dissipation monitoring (QCM-D) sensor to measure in situ interfacial stability and mechanical properties of Candida antarctica Lipase B (CAL B) under varied conditions relating to ring-opening polymerization. The model was fabricated using a dual photochemical approach, where poly(methyl methacrylate) (PMMA) thin films were crosslinked by a photoactive benzophenone monolayer and blended crosslinking agent. This process produces two dimensional, homogenous, rigid PMMA layers, which mimic commercial acrylic resins in a QCM-D experiment. Adsorption of CAL B to PMMA in QCM-D under varied buffer ionic strengths produces a viscoelastic enzyme surface that becomes more rigid as ionic strength increases. The rigid CAL B/PMMA interface demonstrates up to 20 % desorption of enzyme with increasing trace water content. Increased polycaprolactone (PCL) binding at the enzyme surface was also observed, indicating greater PCL affinity for a more hydrated enzyme surface. The enzyme layer destabilized with increasing temperature, yielding near complete reversible catalyst desorption in the model.
Sara V Orski; Santanu Kundu; Richard A Gross; Kathryn L Beers
Related Documents :
8650157 - A gain-of-function of an amyotrophic lateral sclerosis-associated cu,zn-superoxide dism...
9045757 - (-) mating type-specific mutants of phycomyces defective in sex pheromone biosynthesis.
25073397 - Arylsulfatase a bound to poly(butyl cyanoacrylate) nanoparticles for enzyme replacement...
387737 - Genetics and physiology of proline utilization in saccharomyces cerevisiae: enzyme indu...
1341917 - Endopeptidase and carboxypeptidase activities in human urine which hydrolyze bradykinin.
8650157 - A gain-of-function of an amyotrophic lateral sclerosis-associated cu,zn-superoxide dism...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-4
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Implantation of silicon dioxide-based nanocrystalline hydroxyapatite and pure phase beta-tricalciump...
Next Document:  Janus Particles at Walls Modified With Tethered Chains.