| Design and Implementation of Two-Dimensional Polymer Adsorption Models: Evaluating the Stability of Candida antarctica Lipase B/Solid-Support Interfaces by QCM-D. | |
| | |
MedLine Citation:
|
PMID: 23286367 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
A two-dimensional model of a solid-supported enzyme catalyst bead is fabricated on a quartz crystal microbalance with dissipation monitoring (QCM-D) sensor to measure in situ interfacial stability and mechanical properties of Candida antarctica Lipase B (CAL B) under varied conditions relating to ring-opening polymerization. The model was fabricated using a dual photochemical approach, where poly(methyl methacrylate) (PMMA) thin films were crosslinked by a photoactive benzophenone monolayer and blended crosslinking agent. This process produces two dimensional, homogenous, rigid PMMA layers, which mimic commercial acrylic resins in a QCM-D experiment. Adsorption of CAL B to PMMA in QCM-D under varied buffer ionic strengths produces a viscoelastic enzyme surface that becomes more rigid as ionic strength increases. The rigid CAL B/PMMA interface demonstrates up to 20 % desorption of enzyme with increasing trace water content. Increased polycaprolactone (PCL) binding at the enzyme surface was also observed, indicating greater PCL affinity for a more hydrated enzyme surface. The enzyme layer destabilized with increasing temperature, yielding near complete reversible catalyst desorption in the model. |
| | |
Authors:
|
Sara V Orski; Santanu Kundu; Richard A Gross; Kathryn L Beers |
Related Documents
:
|
8650157 - A gain-of-function of an amyotrophic lateral sclerosis-associated cu,zn-superoxide dism... 9045757 - (-) mating type-specific mutants of phycomyces defective in sex pheromone biosynthesis. 387737 - Genetics and physiology of proline utilization in saccharomyces cerevisiae: enzyme indu... 19047657 - Inhibition of inositol phosphorylceramide synthase by the cyclic peptide aureobasidin a. 1341917 - Endopeptidase and carboxypeptidase activities in human urine which hydrolyze bradykinin. 8650157 - A gain-of-function of an amyotrophic lateral sclerosis-associated cu,zn-superoxide dism... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-4 |
Journal Detail:
|
Title: Biomacromolecules Volume: - ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Implantation of silicon dioxide-based nanocrystalline hydroxyapatite and pure phase beta-tricalciump...
Next Document: Janus Particles at Walls Modified With Tethered Chains.