Document Detail


Desiccation tolerance in human cells.
MedLine Citation:
PMID:  11578120     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated for up to 5 days. Here, we have further investigated the factors that allow human cells to survive in the desiccated state. The most important finding is that vacuum greatly enhances the ability of human cells in culture to withstand desiccation. In fact, cells dried slowly and stored under vacuum are able to withstand desiccation even in the absence of added carbohydrates or polyols. In addition to vacuum, the rate of desiccation, the temperature at which cells are maintained, the degree of confluence when dried, and the presence or absence of light have a large effect on the ability to retain viability in the desiccated state. Our data are consistent with a model in which cells can retain viability if they are desiccated in such a way that cellular structures are maintained. However, gradual loss of viability may be due to damage that occurs over time in the desiccated state, perhaps due to free radicals. Further optimization of the process for desiccating and maintaining cells is required before long-term storage of desiccated cells can be achieved.
Authors:
I Puhlev; N Guo; D R Brown; F Levine
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cryobiology     Volume:  42     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-10-01     Completed Date:  2001-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  207-17     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
UCSD Cancer Center, La Jolla, California 92093-0912, USA.
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MeSH Terms
Descriptor/Qualifier:
Carbohydrates / administration & dosage
Cell Line
Cell Survival* / physiology
Colony-Forming Units Assay
Coloring Agents
Desiccation / methods*
Free Radicals / metabolism
Humans
Light
Models, Biological
Polymers / administration & dosage
Temperature
Trehalose / administration & dosage,  metabolism
Trypan Blue
Vacuum
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Coloring Agents; 0/Free Radicals; 0/Polymers; 0/polyol; 72-57-1/Trypan Blue; 99-20-7/Trehalose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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