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Desiccation tolerance among different isolates of the entomopathogenic nematode Steinernema feltiae (Fillipjev).
MedLine Citation:
PMID:  22696940     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Poor storage capacity is a major constraint limiting further expansion of the use of entomopathogenic nematodes. In order to prolong shelf life, a quiescent state of the dauer juveniles (DJs) should be induced. This can be attained by means of desiccation of DJs. In this study, 24 natural isolations of Steinernema feltiae were exposed to desiccation stress in non-ionic polyethylene glycol 600. The dehydrating conditions were measured as water activity, a(w)-value. Non-adapted and adapted DJs were tested separately under a series of dehydrating conditions. The mean tolerated a(w)-value (MW50) ranged from 0.85 for the isolate NEP1 to 0.95 for FIN1, ISR5 and ITA2 when not adapted to desiccation stress and from MW50 of 0.822 for CR1 to 0.98 for ISR6 when adapted to the stress conditions. CR1 tolerated the lowest desiccation stress at an a(w)-value for the most tolerant 10% of the population (MW10) at 0.65 when DJs had been adapted to stress. No significant differences were recorded between all isolates in non-adapted DJs populations MW10 was compared. No correlation between tolerance under non-adapted and adapted conditions were found. Most tolerant isolates will now be used for cross-breeding and subsequent genetic selection to enhance desiccation tolerance.
Authors:
P Nimkingrat; R U Ehlers; O Strauch
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Communications in agricultural and applied biological sciences     Volume:  76     ISSN:  1379-1176     ISO Abbreviation:  Commun. Agric. Appl. Biol. Sci.     Publication Date:  2011  
Date Detail:
Created Date:  2012-06-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101200320     Medline TA:  Commun Agric Appl Biol Sci     Country:  Belgium    
Other Details:
Languages:  eng     Pagination:  293-6     Citation Subset:  IM    
Affiliation:
Christian-Albrechts-University Kiel, Institute for Phytopathology, Dept. for Biotechnology and Biological Control, Hermann-Rodewald Str. 9, 24118 Kiel, Germany.
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