Document Detail

Desiccation as a long-term survival mechanism for the archaeon Methanosarcina barkeri.
MedLine Citation:
PMID:  22194299     Owner:  NLM     Status:  MEDLINE    
Viable methanogens have been detected in dry, aerobic environments such as dry reservoir sediment, dry rice paddies and aerobic desert soils, which suggests that methanogens have mechanisms for long-term survival in a desiccated state. In this study, we quantified the survival rates of the methanogenic archaeon Methanosarcina barkeri after desiccation under conditions equivalent to the driest environments on Earth and subsequent exposure to different stress factors. There was no significant loss of viability after desiccation for 28 days for cells grown with either hydrogen or the methylotrophic substrates, but recovery was affected by growth phase, with cells desiccated during the stationary phase of growth having a higher rate of recovery after desiccation. Synthesis of methanosarcinal extracellular polysaccharide (EPS) significantly increased the viability of desiccated cells under both anaerobic and aerobic conditions compared with that of non-EPS-synthesizing cells. Desiccated M. barkeri exposed to air at room temperature did not lose significant viability after 28 days, and exposure of M. barkeri to air after desiccation appeared to improve the recovery of viable cells compared with that of desiccated cells that were never exposed to air. Desiccated M. barkeri was more resistant to higher temperatures, and although resistance to oxidative conditions such as ozone and ionizing radiation was not as robust as in other desiccation-resistant microorganisms, the protection mechanisms are likely adequate to maintain cell viability during periodic exposure events. The results of this study demonstrate that after desiccation M. barkeri has the innate capability to survive extended periods of exposure to air and lethal temperatures.
Kimberly L Anderson; Ethel E Apolinario; Kevin R Sowers
Related Documents :
3456059 - Induction of hypoxanthine phosphoribosyltransferase deficiency in human u-937 cells.
9394019 - Mutational analysis of regulated exocytosis in tetrahymena.
651939 - Inhibition of post-replication repair by isonicotinic acid hydrazide.
1891779 - 2,4-dichlorophenoxyacetic acid effects on polyamine biosynthesis.
9717179 - Genetic toxicity of n-methylcarbamate insecticides and their n-nitroso derivatives.
1027149 - A cho-cell mutant with a defect in cytokinesis.
12011439 - Clara cell impact in air-side activation of cftr in small pulmonary airways.
1549049 - 13c nmr studies of glucose metabolism in human leukemic cem-c7 and cem-c1 cells.
12644019 - Cycling b-cll cells are highly susceptible to inhibition of the proteasome: involvement...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-12-22
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-02-16     Completed Date:  2012-05-08     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1473-9     Citation Subset:  IM    
University of Maryland Baltimore County, Baltimore, MD, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Methanosarcina barkeri / growth & development,  physiology*
Microbial Viability*
Polysaccharides / metabolism
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resis...
Next Document:  Accurate, rapid taxonomic classification of fungal large-subunit rRNA genes.