| Description of development of rumen ecosystem by PCR assay in milk-fed, weaned and finished lambs in an intensive fattening system. | |
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MedLine Citation:
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PMID: 20050953 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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This study examined the reticulo-rumen characteristics of the microbial community and its fermentative characteristics in milk-fed, at weaning and finished lambs in a conventional fattening system. Five lambs were assigned to each of three groups: milk-fed lambs slaughtered at 30 days (T30), weaned lambs slaughtered at 45 days (T45) and 'finished lambs' slaughtered at 90 days (T90). At slaughter, rumen size, fermentation parameters (pH, volatile fatty acids and microbial enzyme activity) and protozoal counts were recorded. Quantitative PCR was used to quantify the genes encoding 16S and 18S ribosomal DNA of the rumen bacterial and protozoal populations, respectively, and the sequential colonization of the rumen by cellulolytic (Ruminococcus albus, Ruminococcus flavefaciens) and amylolytic (Prevotella ruminicola, Streptococcus bovis) bacteria, and protozoa (Entodinium sp.). Denaturing gradient gel electrophoresis was used to study the development of rumen microbiota biodiversity. Intake of solid food before weaning caused a significant increase in rumen weight (p < 0.0001) and bacterial DNA (p < 0.05) and volatile fatty acid analysis concentration (p < 0.01), whereas pH declined. In milk-fed lambs, cellulolytic bacteria were evident after 30 days. Thereafter, in the 45-day and 90-day groups, the proportions of R. flavefaciens decreased and R. albus increased. Amylolytic bacteria were present in milk-fed lambs; the proportion of P. ruminicola increased in fattening lambs and S. bovis was the least abundant species. Protozoal concentrations were irregular; milk-fed lambs had a significant number of protozoa species from Entodinium and subfamily Isotrichiidae, but they disappeared at weaning. Lamb rumen were refaunated in some individuals at 90 days (Entodinium and subfamily Diplodiniinae spp.), although individual concentrations were variable. |
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Authors:
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A Belanche; J Balcells; G de la Fuente; D R Yañez-Ruíz; M Fondevila; L Calleja |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of animal physiology and animal nutrition Volume: 94 ISSN: 1439-0396 ISO Abbreviation: J Anim Physiol Anim Nutr (Berl) Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101126979 Medline TA: J Anim Physiol Anim Nutr (Berl) Country: Germany |
Other Details:
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Languages: eng Pagination: 648-58 Citation Subset: IM |
Copyright Information:
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© 2009 Blackwell Verlag GmbH. |
Affiliation:
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Departamento de Producción Animal y Ciencia de los Alimentos, Universidad de Zaragoza, C/ Miguel Servet, Zaragoza, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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