Document Detail


Description of a new planktonic mixotrophic dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp. from the coastal waters off Western Korea: morphology, pigments, and ribosomal DNA gene sequence.
MedLine Citation:
PMID:  20487128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mixotrophic dinoflagellate Paragymnodinium shiwhaense n. gen., n. sp. is described from living cells and from cells prepared by light, scanning electron, and transmission electron microscopy. In addition, sequences of the small subunit (SSU) and large subunit (LSU) rDNA and photosynthetic pigments are reported. The episome is conical, while the hyposome is hemispherical. Cells are covered with polygonal amphiesmal vesicles arranged in 16 rows and containing a very thin plate-like component. There is neither an apical groove nor apical line of narrow plates. Instead, there is a sulcal extension-like furrow. The cingulum is as wide as 0.2-0.3 x cell length and displaced by 0.2-0.3 x cell length. Cell length and width of live cells fed Amphidinium carterae were 8.4-19.3 and 6.1-16.0 microm, respectively. Paragymnodinium shiwhaense does not have a nuclear envelope chamber nor a nuclear fibrous connective (NFC). Cells contain chloroplasts, nematocysts, trichocysts, and peduncle, though eyespots, pyrenoids, and pusules are absent. The main accessory pigment is peridinin. The sequence of the SSU rDNA of this dinoflagellate (GenBank AM408889) is 4% different from that of Gymnodinium aureolum, Lepidodinium viride, and Gymnodinium catenatum, the three closest species, while the LSU rDNA was 17-18% different from that of G. catenatum, Lepidodinium chlorophorum, and Gymnodinium nolleri. The phylogenetic trees show that this dinoflagellate belongs within the Gymnodinium sensu stricto clade. However, in contrast to Gymnodinium spp., cells lack nuclear envelope chambers, NFC, and an apical groove. Unlike Polykrikos spp., which have a taeniocyst-nematocyst complex, P. shiwhaense has nematocysts without taeniocysts. In addition, P. shiwhaense does not have ocelloids in contrast to Warnowia spp. and Nematodinium spp. Therefore, based on morphological and molecular analyses, we suggest that this taxon is a new species, also within a new genus.
Authors:
Nam Seon Kang; Hae Jin Jeong; Øjvind Moestrup; Woongghi Shin; Seung Won Nam; Jae Yeon Park; Miguel F De Salas; Ki Woo Kim; Jae Hoon Noh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of eukaryotic microbiology     Volume:  57     ISSN:  1550-7408     ISO Abbreviation:  J. Eukaryot. Microbiol.     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-21     Completed Date:  2010-08-13     Revised Date:  2010-11-10    
Medline Journal Info:
Nlm Unique ID:  9306405     Medline TA:  J Eukaryot Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  121-44     Citation Subset:  IM    
Affiliation:
School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AM408889
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MeSH Terms
Descriptor/Qualifier:
Chloroplasts / chemistry
Cluster Analysis
DNA, Protozoan / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Dinoflagellida / classification*,  cytology,  genetics,  isolation & purification*
Genes, rRNA
Microscopy
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular Sequence Data
Phylogeny
Pigments, Biological / analysis
RNA, Protozoan / genetics
RNA, Ribosomal / genetics
RNA, Ribosomal, 18S / genetics
Republic of Korea
Seawater / parasitology*
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Protozoan; 0/DNA, Ribosomal; 0/Pigments, Biological; 0/RNA, Protozoan; 0/RNA, Ribosomal; 0/RNA, Ribosomal, 18S; 0/RNA, ribosomal, 26S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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