Document Detail


Dermatan sulfate is the major metachromatic glycosaminoglycan in the integument of the anuran Bufo ictericus.
MedLine Citation:
PMID:  17137817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glycosaminoglycans from the ventral and dorsal integuments of the anuran Bufo ictericus were characterized based on biochemical and histochemical methods. Dermatan sulfate is the major metachromatic glycosaminoglycan found in these tissues, but small amount of heparan sulfate was also detected. The average molecular mass of the dermatan sulfate is approximately 20 kDa, similar to the glycosaminoglycan isolated from mammalian skin. In addition, the amphibian integument contains high amounts of hyaluronic acid, especially in the ventral area. We also observed that the glycosaminoglycans occur in the anuran integument as irregular deposits through the spongious dermis and in the mast cells, as revealed by histochemical analysis using Alcian blue, dimethylmethylene blue and toluidine blue stains. The concentration and composition of glycosaminoglycans found in the amphibian integument resemble those from mammalian skin except for the higher concentration of hyaluronic acid in the amphibian tissue. Possibly, this observation indicates that the function of the sulfated glycosaminoglycan in these tissues has been preserved during evolution, although the amphibian integument and the human skin have their own particular physiology.
Authors:
A A Pelli; R A Azevedo; L P Cinelli; P A S Mourão; L de Brito-Gitirana
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-24
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  146     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-02-06     Completed Date:  2007-07-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  160-5     Citation Subset:  IM    
Affiliation:
Laboratório de Histologia Animal e Comparada, Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, RJ, Brazil, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ 21540-970, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bufonidae*
Dermatan Sulfate / analysis*,  chemistry
Electrophoresis, Polyacrylamide Gel
Glycosaminoglycans / analysis*,  chemistry
Hyaluronic Acid / analysis,  chemistry
Male
Molecular Weight
Skin / chemistry*
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 24967-94-0/Dermatan Sulfate; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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