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Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of F (0) fluorescence in leaves of Arabidopsis thaliana and Zea mays.
MedLine Citation:
PMID:  23196877     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effect of stepwise increments of red light intensities on pulse-amplitude modulated (PAM) chlorophyll (Chl) fluorescence from leaves of A. thaliana and Z. mays was investigated. Minimum and maximum fluorescence were measured before illumination (F (0) and F (M), respectively) and at the end of each light step ([Formula: see text] and [Formula: see text], respectively). Calculated [Formula: see text] values derived from F (0), F (M) and [Formula: see text] fluorescence according to Oxborough and Baker (1997) were lower than the corresponding measured [Formula: see text] values. Based on the concept that calculated [Formula: see text] values are under-estimated because the underlying theory ignores PSI fluorescence, a method was devised to gain relative PSI fluorescence intensities from differences between calculated and measured [Formula: see text]. This method yields fluorometer-specific PSI data as its input data (F (0), F (M), [Formula: see text] and [Formula: see text]) depend solely on the spectral properties of the fluorometer used. Under the present conditions, the PSI contribution to F (0) fluorescence was 0.24 in A. thaliana and it was independent on the light acclimation status; the corresponding value was 0.50 in Z. mays. Correction for PSI fluorescence affected Z. mays most: the linear relationship between PSI and PSII photochemical yields was clearly shifted toward the one-to-one proportionality line and maximum electron transport was increased by 50 %. Further, correction for PSI fluorescence increased the PSII reaction center-specific parameter, 1/F (0) - 1/F (M), up to 50 % in A. thaliana and up to 400 % in Z. mays.
Authors:
Erhard E Pfündel; Christof Klughammer; Armin Meister; Zoran G Cerovic
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-30
Journal Detail:
Title:  Photosynthesis research     Volume:  -     ISSN:  1573-5079     ISO Abbreviation:  Photosyn. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954728     Medline TA:  Photosynth Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Lehrstuhl für Botanik II der Universität Würzburg, Julius-von-Sachs Institut für Biowissenschaften, 97082, Würzburg, Germany, epfuendel@walz.com.
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