| Derivatization of controlled pore glass beads for solid phase oligonucleotide synthesis. | |
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MedLine Citation:
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PMID: 3273639 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved and simplified procedure for the attachment of nucleosides onto long chain alkylamine controlled pore glass beads (LCAA-CPG) is presented. This procedure uses 1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (DEC) to couple nucleoside 3'-succinates directly to the LCAA-CPG. The preparation of nucleoside 3'-succinate anhydrides, p-nitrophenyl, or pentachlorophenyl esters and the use of highly toxic dicyclohexylcarbodiimide (DCC) is no longer required. Procedures involving acidic activation of the LCAA-CPG before derivatization and a pre-synthesis capping are also described, which prevent the formation of oligonucleotides linked by 3'-phosphates to the LCAA-CPG. Evidence is presented indicating that this type of linkage is responsible for the apparently greater than 100% coupling yields observed for the first coupling cycle. |
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Authors:
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R T Pon; N Usman; K K Ogilvie |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: BioTechniques Volume: 6 ISSN: 0736-6205 ISO Abbreviation: BioTechniques Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1990-06-06 Completed Date: 1990-06-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8306785 Medline TA: Biotechniques Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 768-75 Citation Subset: IM |
Affiliation:
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Dept. of Medical Biochemistry, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Colorimetry Esters / chemical synthesis Glass Nucleosides / chemical synthesis Oligonucleotides / chemical synthesis* Receptor Aggregation Succinates / chemical synthesis |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Nucleosides; 0/Oligonucleotides; 0/Succinates |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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