| Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives. | |
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MedLine Citation:
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PMID: 11355824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have been discussed in detail: (i) the mol ratios of the OPA-SH-group-containing additive amino acid; (ii) the preparation and storage conditions of the OPA reagents; (iii) the optimum pH conditions for the interactions and elutions; (iv) the behavior of the, believed to be, less stable amino acids, such as glycine, beta-alanine, gamma-aminobutyric acid, histidine, ornithine and lysine. |
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Authors:
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I Molnár-Perl |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Journal of chromatography. A Volume: 913 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-05-17 Completed Date: 2001-09-20 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 283-302 Citation Subset: IM |
Affiliation:
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Institute of Inorganic and Analytical Chemistry, L. Eötvös University, Budapest, Hungary. perlne@para.chem.elte.hu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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physiology* Chromatography, High Pressure Liquid / methods Sulfhydryl Compounds / chemistry* o-Phthalaldehyde / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Sulfhydryl Compounds; 643-79-8/o-Phthalaldehyde |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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