Document Detail

Derivatives of [3H]serotonin in organ cultures of hamster pineal gland.
MedLine Citation:
PMID:  6203004     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine the viability of the hamster pineal gland in organ culture and to test the effect of norepinephrine (NE) on [3H]serotonin derivatives. In this study, elevated levels of melatonin (7-fold, p less than .05), 5- hydroxytrytophol (5-fold, p less than .001), 5-methoxytryptophol (1.78-fold, p less than .05), and depressed levels of 5-hydroxyindoleacetic acid (3.8-fold, p less than .02) and methoxyindoleacetic acid (1.78-fold, p less than .05) were detected in the glands following the addition of NE to the medium. In a separate experiment, melatonin concentration in the media was also periodically measured by radioimmunoassay to determine the viability of the organ culture over a four-day period. The melatonin level on day 2 (2321 +/- 106 pg/gland) was significantly higher (p less than 0.01) than on day 3 (1542 +/- 86 pg/gland) or day 4 (805 +/- 39 pg/gland). The results of these experiments verify the viability of the hamster pineal organ culture and show that the gland responds to NE in vitro.
C M Craft; R J Reiter
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  34     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-06-27     Completed Date:  1984-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1775-82     Citation Subset:  IM    
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MeSH Terms
Chromatography, Thin Layer
Hydroxyindoleacetic Acid / analogs & derivatives,  metabolism
Melatonin / biosynthesis*
Norepinephrine / physiology
Organ Culture Techniques
Pineal Gland / metabolism*
Serotonin / metabolism*
Time Factors
Reg. No./Substance:
3471-31-6/5-methoxyindoleacetic acid; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 54-16-0/Hydroxyindoleacetic Acid; 73-31-4/Melatonin

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