| Derivation of type II alveolar epithelial cells from murine embryonic stem cells. | |
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MedLine Citation:
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PMID: 12201994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Embryonic stem (ES) cell pluripotency is being investigated increasingly to obtain specific cell lineages for tissue engineering. However, the possibility that ES cells can give rise to lung tissue has not been tested. We hypothesized that lung epithelial cells (type II pneumocytes) can be derived in vitro from murine ES cells. After withdrawal of leukemia inhibitory factor (LIF) and formation of embryoid bodies in maintenance medium for 10, 20, and 30 days, differentiating ES cells were kept in the same medium or transferred to serum-free small airway growth medium (SAGM) for a further 3 or 14 days of culture. The presence of type II pneumocytes in the resulting mixed cultures was demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR) of surfactant protein C (SPC) mRNA, immunostaining of SPC, and electron microscopy of osmiophilic lamellar bodies only at 30 days sampling time. SAGM appeared to be more favorable for type II cell formation than ES medium. No SPC transcripts were found in differentiating cells grown under the same conditions without formation of embryoid bodies. These findings could form the basis for the enrichment of ES cell-derived cultures with type II pneumocytes, and provide an in vitro system for investigating mechanisms of lung repair and regeneration. |
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Authors:
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Nadire N Ali; Alasdair J Edgar; Ali Samadikuchaksaraei; Catherine M Timson; Hanna M Romanska; Julia M Polak; Anne E Bishop |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Tissue engineering Volume: 8 ISSN: 1076-3279 ISO Abbreviation: Tissue Eng. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-30 Completed Date: 2003-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9505538 Medline TA: Tissue Eng Country: United States |
Other Details:
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Languages: eng Pagination: 541-50 Citation Subset: IM |
Affiliation:
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Tissue Engineering Centre, Imperial College Faculty of Medicine, Chelsea & Westminster Hospital, London, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers Cell Differentiation Epithelial Cells / cytology*, metabolism, ultrastructure Immunohistochemistry Mice Microscopy, Phase-Contrast Pluripotent Stem Cells / cytology Pulmonary Alveoli / cytology* Pulmonary Surfactants / metabolism RNA, Messenger Stem Cells / cytology*, metabolism, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Pulmonary Surfactants; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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