Document Detail


Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos.
MedLine Citation:
PMID:  20177993     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we focused on the derivation of human embryonic stem cell (hESC) from preimplantation genetic screening (PGS)-analyzed and preimplantation genetic diagnosis (PGD)-analyzed embryos. Out of 62 fresh PGD/PGS-analyzed embryos, 22 embryos reached the blastocyst stage. From 12 outgrowth blastocysts, we derived four hESC lines onto a feeder layer. Surprisingly, karyotype analysis showed that hESC lines derived from aneuploid embryos had diploid female karyotype. One hESC line was found to carry a balanced Robertsonian translocation. All the cell lines showed hESC markers and had the pluripotent ability to differentiate into derivatives of the three embryonic germ layers. The established lines had clonal propagation with 22-31% efficiency in the presence of ROCK inhibitor. These results further indicate that hESC lines can be derived from PGD/PGS-analyzed embryos that are destined to be discarded and can serve as an alternative source for normal euploid lines.
Authors:
Adeleh Taei; Hamid Gourabi; Ali Seifinejad; Mehdi Totonchi; Ebrahim Shahbazi; Mojtaba Rezazadeh Valojerdi; Poopak Eftekhari; Leila Karimian; Hossein Baharvand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-23
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  395-402     Citation Subset:  IM    
Affiliation:
Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box 19395-4644, Tehran, Iran.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastomeres / cytology
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Colony-Forming Units Assay
Embryo, Mammalian / cytology*
Embryonic Stem Cells / cytology*
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Mice
Preimplantation Diagnosis / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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