| Derivation and induction of the differentiation of animal ES cells as well as human pluripotent stem cells derived from fetal membrane. | |
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MedLine Citation:
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PMID: 17022145 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We succeeded in the derivation and maintenance of pluripotent embryonic stem (ES) cells from equine and bovine blastocysts. These cells expressed markers that are characteristics of mouse ES cells, namely, alkaline phosphatase, stage-specific embryonic antigen 1, STAT 3 and Oct 4. We confirmed the pluripotential ability of these cells, which were able to undergo somatic differentiation in vitro to neural progenitors and to endothelial or hematopoietic lineages. We were able to use bovine ES cells as a source of nuclei for nuclear transfer and we generated cloned cattle with a higher frequency of pregnancies to term than has been achieved with somatic cells. On the other hand, we established human fetal membrane derived stem cell lines by the colonial cloning techniques using MEMalpha culture medium containing 10 ng/ml of EGF, 10 ng/ml of LIF and 10% fetal bovine serum (FBS). These cells appeared to maintain normal karyotype in vitro and expressed markers characteristics of stem cells. Furthermore, these cells contributed to the formation of chimeric murine embryoid bodies and gave rise to all three germ layers in vitro. Results from animal ES cells and human fetal membrane derived stem cells clearly demonstrate that these cells might be used for providing different types of cells for regenerative medicine as well as used for targeted genetic manipulation of the genome. |
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Authors:
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Shigeo Saito; Kazunari Yokoyama; Tomoharu Tamagawa; Isamu Ishiwata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Human cell Volume: 18 ISSN: 0914-7470 ISO Abbreviation: Hum. Cell Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2006-10-06 Completed Date: 2006-12-08 Revised Date: 2012-05-31 |
Medline Journal Info:
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Nlm Unique ID: 8912329 Medline TA: Hum Cell Country: Japan |
Other Details:
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Languages: eng Pagination: 135-41 Citation Subset: IM |
Affiliation:
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Saito Laboratory of Cell Technology, 2095-20 Kataoka, Yaita, Tochigi 329-1571, Japan. saict1@maple.ocn.ne.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Differentiation* Embryonic Stem Cells / cytology* Extraembryonic Membranes / cytology* Horses Humans Mice Pluripotent Stem Cells / cytology* Regenerative Medicine |
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