Document Detail


Derivation of induced pluripotent stem cells from fetal human skin fibroblasts.
MedLine Citation:
PMID:  22649648     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The isolation and study of autologous human stem cells remain among the most urgent problems in cell biology and biomedicine to date. Induced pluripotent stem cells can be derived from human somatic cells by the overexpression of a number of genes. In this study we reprogrammed fetal human skin fibroblasts by transduction with retroviral vectors carrying murine Oct4 , Sox2 , Klf4 , and c-Myc cDNAs. As a result, cells with the protein expression and gene transcription pattern characteristic of human embryonic stem cells were derived. These induced pluripotent cells are capable of differentiation in vitro into the ectoderm, mesoderm, and endoderm derivatives.
Authors:
S P Medvedev; A A Malakhova; E V Grigor'eva; A I Shevchenko; E V Dementyeva; I A Sobolev; I N Lebedev; A G Shilov; I F Zhimulev; S M Zakian
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta naturae     Volume:  2     ISSN:  2075-8251     ISO Abbreviation:  Acta Naturae     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2012-05-31     Completed Date:  2012-08-23     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101525823     Medline TA:  Acta Naturae     Country:  Russia (Federation)    
Other Details:
Languages:  eng     Pagination:  102-6     Citation Subset:  -    
Affiliation:
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences.
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