| Derivation and characterization of human embryonic germ cells: serum-free culture and differentiation potential. | |
| | |
MedLine Citation:
|
PMID: 19712561 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study examined the effects of a chemically defined culture medium supplement, knock-out serum replacement (KSR), on the growth and differentiation of human embryonic germ cells (hEgc) and found that the efficiency of the initial establishment of hEGC lines in KSR medium was significantly higher than in fetal calf serum (FCS) medium. The percentage of undifferentiated hEGC colonies growing in KSR medium was significantly higher than in FCS-based medium (P < 0.05). The hEGC colonies showed typical mouse embryonic germ cell-like morphology. They showed normal and stable diploid karyotype and expressed alkaline phosphatase (AP), stage-specific embryonic antigens (SSEA) and other specific markers of pluripotent cells. In addition, hEGC could form simple and cystic embryoid bodies (EB) that consisted of various cell types including neural, epithelial and rhythmically beating cardiac cells, even sperm-like and oocyte-like cells. Tumour-like outgrowths were formed in nude mice and found to contain a variety of cell types, including uterine epithelium, adipocytes, squamous tissue and skin structures. In conclusion, an appropriate serum-free culture system has been developed for the establishment of hEGC lines. This may provide an in-vitro model to study differentiation and can be used as a potential source of therapy for infertility and regenerative medicine. |
| | |
Authors:
|
Jinlian Hua; Haisheng Yu; Sheng Liu; Zhongying Dou; Yadong Sun; Xiaoqi Jing; Chunrong Yang; Anmin Lei; Huayan Wang; Zhimin Gao |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Reproductive biomedicine online Volume: 19 ISSN: 1472-6491 ISO Abbreviation: Reprod. Biomed. Online Publication Date: 2009 Aug |
Date Detail:
|
Created Date: 2009-08-28 Completed Date: 2009-11-09 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101122473 Medline TA: Reprod Biomed Online Country: England |
Other Details:
|
Languages: eng Pagination: 238-49 Citation Subset: IM |
Affiliation:
|
College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology, Shaanxi Key Lab for Agriculture Molecular Biotechnology Centre, Northwest A and F University, Yangling, Shaanxi, 712100 China. jlhua2003@126.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Cell Differentiation* Cells, Cultured Culture Media Culture Media, Serum-Free DNA Primers Germ Cells / cytology* Humans Immunohistochemistry Karyotyping Mice Microscopy, Electron, Scanning Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Culture Media, Serum-Free; 0/DNA Primers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comparative genomic hybridization of oocytes and first polar bodies from young donors.
Next Document: Long-term follow-up after cervical cancer treatment and subsequent successful surrogate pregnancy.