Document Detail

Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark.
MedLine Citation:
PMID:  20198448     Owner:  NLM     Status:  MEDLINE    
The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank (
Linda Harkness; Iben Anne Rasmussen; Karin Erb; Moustapha Kassem
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-03
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-19     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  259-68     Citation Subset:  IM    
Molecular Endocrinology laboratory (KMEB), Medical Biotechnology Centre, University of South Denmark, Winslowparken 25, 1 sal, 5000 Odense, Denmark.
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MeSH Terms
Blastocyst / cytology
Cell Culture Techniques / methods*
Cell Differentiation / genetics
Cell Line
Colony-Forming Units Assay
Embryo Research*
Embryonic Stem Cells / cytology*
Reverse Transcriptase Polymerase Chain Reaction
Teratoma / pathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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