Document Detail


Derepression of HPRT locus on inactive X chromosome of human lymphoblastoid cell line.
MedLine Citation:
PMID:  3466359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human XX lymphoblastoid cells with a deletion in the HPRT locus on the active X were exposed to HPRT clone pHPT32. HPRT+ isolates GPT3 and GPT5 lacked pHPT32 DNA, suggesting that their HPRT+ phenotype resulted from expression of a cellular gene. GPT3 mutated to thioguanine resistance at least 100 times more frequently than cells in which the expressed HPRT locus was on the active X. Most GPT3-derived HPRT- had lost one entire X chromosome, indicating that the HPRT+ phenotype of GPT3 resulted from derepression of the HPRT locus on its inactive X. Virtually unchanged G6PD and PGK activities and the presence of a late-replicating X in GPT3 suggest that derepression of the inactive X was not general. Eleven of the GPT3-derived mutants had a tiny centric remnant that may result from a frequently operative mechanism of X chromosome loss. The detection of partial or complete loss of an X by direct selection presents unusual opportunities for genotoxicity detection with human cells.
Authors:
N Nadon; G Sekhon; L J Brown; N Korn; J W Petersen; J Strandtmann; C Chang; R DeMars
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Somatic cell and molecular genetics     Volume:  12     ISSN:  0740-7750     ISO Abbreviation:  Somat. Cell Mol. Genet.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  1987-01-14     Completed Date:  1987-01-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8403568     Medline TA:  Somat Cell Mol Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  541-54     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Chromosome Banding
Chromosome Deletion
Cloning, Molecular
Enzyme Repression
Genes*
Humans
Hypoxanthine Phosphoribosyltransferase / biosynthesis,  genetics*
Karyotyping
Leukemia, Lymphoid
Mutation
Nucleic Acid Hybridization
X Chromosome*
Grant Support
ID/Acronym/Agency:
AI15486/AI/NIAID NIH HHS; GM06983/GM/NIGMS NIH HHS; P30-CA14520/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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