Document Detail

Deregulation of catalase, not MnSOD, is associated with necrotic death of p53-defective DF-1 cells under antimycin A-induced oxidative stress.
MedLine Citation:
PMID:  15528999     Owner:  NLM     Status:  MEDLINE    
One of distinct genetic alterations in spontaneously immortalized DF-1 cells was found to be dysfunction of p53 and E2F-1 as well as altered antioxidant gene expression (upregulation of MnSOD and downregulation of catalase). We have characterized the cellular responses of primary and immortal DF-1 cells to oxidative stress and found that DF-1 cells were more sensitive to oxidative stress than their primary counterparts when treated with antimycin A. The increased DF-1 cell death by oxidative stress was accompanied by an increase in the levels of intracellular superoxide anions and hydrogen peroxide. The cell death in DF-1 cells by antimycin A showed none of the hallmarks of apoptosis, but displayed a significantly increased necrotic cell population. Anti-apoptotic Bcl-2 failed to inhibit oxidative-induced necrotic cell death in the DF-1 cells. However, this necrotic cell death was significantly decreased by treatment with hydrogen peroxide scavengers such as sodium pyruvate and N-acetyl-cysteine. Interestingly, overexpression of human catalase in DF-1 cells endowed cells resistant to the oxidative stress by antimycin A treatment, although the downregulation of MnSOD by an antisense strategy showed no evident change in the cytotoxic effect caused by antimycin A. Taken together, the present study might provide new therapeutic approach for tumor cells having the loss of p53 function and the altered antioxidant functions.
Seungkwon You; Byung-Whi Kong; Sun-Young Jeon; Douglas N Foster; Hyunggee Kim
Related Documents :
15996779 - Fraxetin prevents rotenone-induced apoptosis by induction of endogenous glutathione in ...
11755319 - Toxicity by pyruvate in hepg2 cells depleted of glutathione: role of mitochondria.
8831699 - Peroxynitrite cytotoxicity on bovine retinal pigmented epithelial cells in culture.
10048749 - Reduced levels of catalase activity potentiate mpp+-induced toxicity: comparison betwee...
1333309 - Variable alpha-tocopherol stimulation and protection of glutathione peroxidase activity...
10777699 - Monoamine oxidase b induces erk-dependent cell mitogenesis by hydrogen peroxide generat...
24317439 - The hsp90 inhibitor 17-n-allylamino-17-demethoxy geldanamycin (17-aag) synergizes with ...
10964749 - Acylphosphatase is a strong apoptosis inducer in hela cell line.
16092729 - Direct exposure methods for testing native atmospheres.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecules and cells     Volume:  18     ISSN:  1016-8478     ISO Abbreviation:  Mol. Cells     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-05     Completed Date:  2005-04-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9610936     Medline TA:  Mol Cells     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  220-9     Citation Subset:  IM    
Laboratory of Cell Growth and Function Regulation, College of Life and Environmental Sciences, Korea University, Seoul 136-701, Korea.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antimycin A / pharmacology
Catalase / genetics,  physiology*
Cell Line
Chick Embryo
Fibroblasts / metabolism,  pathology*
Free Radical Scavengers / pharmacology
Gene Expression Regulation, Enzymologic / drug effects,  physiology*
Oxidative Stress*
Superoxide Dismutase / genetics,  physiology*
Tumor Suppressor Protein p53 / deficiency*
Reg. No./Substance:
0/Free Radical Scavengers; 0/Tumor Suppressor Protein p53; 642-15-9/Antimycin A; EC; EC Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cysteine-321 of human brain GABA transaminase is involved in intersubunit cross-linking.
Next Document:  Precocious expression of Drosophila Rbp9 inhibits ovarian germ cell proliferation.