Document Detail


Deprival of testicular innervation induces apoptosis of Leydig cells via caspase-8-dependent signaling: a novel survival pathway revealed.
MedLine Citation:
PMID:  19268429     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leydig cells are the primary source of testosterone in adult males. Recently, a growing body of evidence has shown that testicular innervation functions as a major regulator in Leydig cell steroidogenesis. The question then arises whether this novel regulatory pathway also plays an important role in other biological behaviors of this cell type. In the present study, we selectively resected the superior spermatic nerves (SSNs) or the inferior spermatic nerves (ISNs) to investigate the effects of testicular denervation on survival of Leydig cells. After testicular denervation, Leydig cells displayed morphological characteristics of apoptosis, such as chromatin condensation, cell shrinkage and apoptotic body formation. Flow cytometry combined with TUNEL labeling demonstrated dramatic and persistent apoptosis of Leydig cells in the denervated testes 14 and 21 days after operation. Meanwhile, serum T concentrations in the SSN- or ISN-denervated rats dramatically decreased on day 14 and declined further on day 21. Plasma LH levels underwent a remarkable rise, while serum FSH levels remained unchanged. Immunofluorescent staining and flow cytometry further demonstrated that testicular denervation activated caspase-3 and caspase-8, but not caspase-9 in Leydig cells. Our data indicate that testicular innervation functions as an important survival factor for Leydig cells in vivo.
Authors:
Yong-Guang Gong; Yi-Qiu Wang; Min Gu; Ming-Ming Feng; Wei Zhang; Ren-Shan Ge
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-03-04
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  382     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-05-12     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  165-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Caspase 8 / metabolism*
Cell Survival
Denervation
Follicle Stimulating Hormone / blood
Leydig Cells / cytology,  enzymology,  physiology*
Luteinizing Hormone / blood
Male
Rats
Rats, Sprague-Dawley
Signal Transduction
Testis / cytology,  innervation*
Grant Support
ID/Acronym/Agency:
R01 AG030598/AG/NIA NIH HHS; R01 AG030598/AG/NIA NIH HHS; R01 AG030598-02/AG/NIA NIH HHS; R01 HD050570/HD/NICHD NIH HHS; R01 HD050570/HD/NICHD NIH HHS; R01 HD050570-02/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone; EC 3.4.22.-/Caspase 8
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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