| Depot-specific differences in the insulin response of adipose-derived stromal cells. | |
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MedLine Citation:
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PMID: 20599584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Visceral adiposity is more strongly linked to insulin resistance than subcutaneous adiposity. High insulin levels can be mitogenic or adipogenic to adipocytes, but little is known regarding these effects of insulin on stromal cells from visceral and subcutaneous fat depots. Consequently, we measured adipogenesis and mitosis in response to elevated insulin levels in rat adipose-derived stromal cells (ADSCs) from visceral (perirenal) and subcutaneous depots. Insulin alone, at 10 microM, did not stimulate adipogenesis in naïve perirenal visceral (pvADSCs) or subcutaneous ADSCs (scADSCs), although a significant increase in proliferation occurred in both. Adipogenesis, induced using adipocyte differentiation medium (AM), resulted in greater lipid accumulation in pvADSCs, but the associated decrease in proliferation was less than in scADSCs. Omission of insulin from AM significantly reduced lipid accumulation in pvADSCs, but had little effect in scADSC, whilst proliferation was inhibited more in scADSCs than pvADSCs. Consequently, insulin is more lipogenic and less mitogenic in differentiating pvADSCs compared to scADSCs. |
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Authors:
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H Sadie-Van Gijsen; N J Crowther; F S Hough; W F Ferris |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-25 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 328 ISSN: 1872-8057 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2011-01-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 22-7 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Division of Endocrinology, Department of Medicine, Faculty of Health Sciences, University of Stellenbosch, Francie van Zijl Drive, Tygerberg 7505, South Africa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipogenesis
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drug effects,
genetics,
physiology Adipose Tissue / cytology, drug effects*, metabolism, physiology Animals Body Fat Distribution Cell Culture Techniques Cell Differentiation / drug effects, genetics Cell Proliferation / drug effects Cells, Cultured Insulin / pharmacology* Lipid Metabolism / drug effects, genetics Male Organ Specificity / physiology Rats Rats, Wistar Stromal Cells / drug effects*, metabolism, physiology Time Factors |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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