Document Detail

Deposition, turnover, and release of CENH3 at Arabidopsis centromeres.
MedLine Citation:
PMID:  21842230     Owner:  NLM     Status:  Publisher    
The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres varies between different organisms. We confirmed by in vivo studies that deposition of Arabidopsis CENH3 takes place at centromeres during G2 and demonstrated that additionally a low turnover of CENH3 occurs along the cell cycle, apparently for replacement of damaged protein. Furthermore, enhanced yellow fluorescent protein (EYFP)-CENH3 of photobleached chromocenters is not replaced by EYFP-CENH3 molecules from unbleached centromeres of the same nucleus, indicating a stable incorporation of CENH3 into centromeric nucleosomes. In differentiated endopolyploid nuclei however, the amount of CENH3 at centromeres declines with age.
Inna Lermontova; Twan Rutten; Ingo Schubert
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-13
Journal Detail:
Title:  Chromosoma     Volume:  -     ISSN:  1432-0886     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466, Gatersleben, Germany,
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