| Deposition and mobilization of cholesterol ester in cultured human skin fibroblasts. | |
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MedLine Citation:
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PMID: 1009094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human skin fibroblasts in culture served as a model system to study intracellular cholesterol ester deposition in mesenchymal cells. Confluent cultures were exposed to homologous low density lipoprotein alone and together with chloroquine. In the presence of low density lipoprotein alone, even at half circulating serum concentrations, cellular free cholesterol increased no more than 12%, while the increase in cholesterol ester ranged from 13--100% during 48 h of incubation. Addition of chloroquine to the culture medium containing low density lipoprotein resulted in a very marked increase in cholesterol ester and the ratio of cellular esterified cholesterol to free cholesterol rose up to 2.2. In the presence of chloroquine the sum of uptake and degradation of 125I-labeled low density lipoprotein was enhanced and at higher chloroquine concentrations the more pronounced inhibition of degradation resulted in the intracellular retention of undegraded protein. Upon removal of the chloroquine-containing medium, there was a slight fall in the cellular cholesterol after 24 h incubation in a medium containing 10% fetal calf serum. Replacement of the fetal calf serum by lipoprotein-deficient serum and a mixture of high density apolipoprotein and sphingomyelin increased very significantly the loss of total cholesterol from the cells. At the same time the rate of loss of the retained labeled low density lipoprotein was also increased. The present results provide an adequate and reproducible model system for the study of cholesterol accumulation in human mesenchymal cells, which is one of the basic changes in atheromatosis. The availability of cholesterol ester laden cells also provides a good system for the study of agents active in cholesterol removal. |
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Authors:
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O Stein; J Vanderhoek; G Friedman; Y Stein |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 450 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1976 Dec |
Date Detail:
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Created Date: 1977-03-21 Completed Date: 1977-03-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 367-78 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Apolipoproteins / metabolism Biological Transport Cells, Cultured Chloroquine / pharmacology Cholesterol / analogs & derivatives*, metabolism Cholesterol Esters / biosynthesis, metabolism* Fibroblasts / drug effects, metabolism Humans Lipoproteins / metabolism Liposomes / metabolism Male Skin / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/Cholesterol Esters; 0/Lipoproteins; 0/Liposomes; 54-05-7/Chloroquine; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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