Document Detail


Depolymerization of cytokeratin intermediate filaments faciliates the intracelullar infection of Bartonella henselae in HeLa cell.
MedLine Citation:
PMID:  23359593     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bartonella henselae (Bh) is capable of invading epithelial and endothelial cells through the modulation of the function of actin-dependent cytoskeleton. Although understanding of the pathogenesis has been increased by the development of an infection model in endothelial cells in vitro, little is known about the interactional mechanism of Bh infection with epithelial cells. This study aims to identify the binding candidates of Bh infection in epithelial cells and explores their effect on Bh infection. Pull-down assays and mass spectrometry analysis confirmed that some of the binding proteins are cytoskeleton associated (keratin 14, keratin 6, F-actin). Bh infection significantly induces the expression of the cytokeratin genes. Chemical disruption of the keratin network by using ethylene glycol bis (2-aminoethyl) tetraacetic acid (EGTA) promotes the intracellular persistence of Bh in HeLa cells. However, cytochalasin B and phalloidin treatment inhibits Bh invasion. Immunofluorescent staining demonstrates that Bh infection induces an F-actin-dependent rearrangement of the cytoskeleton. However, we demonstrated that keratin intermediate filaments (IFs) are depolymerized by Bh infection, using immunofluorescent staining and whole-mount cell electron microscopy. The results indicate that Bh achieves an intracellular persistence in epithelial cells through the depolymerization of cytokeratin IFs that are protective against Bh invasion.
Authors:
Caixia Zhu; Yajie Bai; Qiyong Liu; Dongmei Li; Jiehua Hong; Zhibiao Yang; Li Cui; Xiuguo Hua; Congli Yuan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-28
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  -     ISSN:  1537-6613     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, P.R China.
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