| Depolarisation of the plasma membrane in the arsenic trioxide (As2O3)-and anti-CD95-induced apoptosis in myeloid cells. | |
| | |
MedLine Citation:
|
PMID: 15581621 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Depolarisation of the plasma membrane has been shown to be actively regulated during lymphocyte-apoptosis. Here, we present data about anti-Fas and As2O3 induced depolarisation of myeloid U-937 cells. Anti-Fas but not As2O3-induced depolarisation was significantly dependent on caspase-activation. Na+-fluxes contributed to the depolarisation in early stages of As2O3-induced apoptosis, whereas the membrane potential in late stages depended on Cl- -fluxes. Cl- -channels also played an important role in the induction of cell shrinkage in As2O3-induced apoptosis. However, none of these ions contributed significantly to anti-Fas induced depolarisation. This indicates the existence of different mechanisms for apoptotic plasma membrane depolarisation within one cell type. |
| | |
Authors:
|
Florian Nolte; Oliver Friedrich; Markus Rojewski; Rainer H A Fink; Hubert Schrezenmeier; Sixten Körper |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: FEBS letters Volume: 578 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-12-07 Completed Date: 2005-02-15 Revised Date: 2005-09-01 |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 85-9 Citation Subset: IM |
Affiliation:
|
Department of Transfusion Medicine, University of Ulm, Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
/
pharmacology Antibodies, Monoclonal / metabolism Antigens, CD95 / immunology, metabolism* Antineoplastic Agents / pharmacology* Apoptosis / physiology* Arsenicals / pharmacology* Cell Membrane / metabolism* Chloride Channels / metabolism Chlorides / metabolism Humans Membrane Potentials / drug effects, physiology* Myeloid Cells* / cytology, drug effects, physiology Nitrobenzoates / pharmacology Oxides / pharmacology* Sodium / metabolism U937 Cells |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/Antineoplastic Agents; 0/Arsenicals; 0/Chloride Channels; 0/Chlorides; 0/Nitrobenzoates; 0/Oxides; 0/anti-Fas monoclonal antibody; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 1327-53-3/arsenic trioxide; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 7440-23-5/Sodium |
| Comments/Corrections | |
Erratum In:
|
FEBS Lett. 2005 Jul 4;579(17):3866 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Glutamate-evoked redox state alterations are involved in tissue transglutaminase upregulation in pri...
Next Document: Protein kinase Akt/PKB phosphorylates heme oxygenase-1 in vitro and in vivo.