Document Detail

Depolarisation of the plasma membrane in the arsenic trioxide (As2O3)-and anti-CD95-induced apoptosis in myeloid cells.
MedLine Citation:
PMID:  15581621     Owner:  NLM     Status:  MEDLINE    
Depolarisation of the plasma membrane has been shown to be actively regulated during lymphocyte-apoptosis. Here, we present data about anti-Fas and As2O3 induced depolarisation of myeloid U-937 cells. Anti-Fas but not As2O3-induced depolarisation was significantly dependent on caspase-activation. Na+-fluxes contributed to the depolarisation in early stages of As2O3-induced apoptosis, whereas the membrane potential in late stages depended on Cl- -fluxes. Cl- -channels also played an important role in the induction of cell shrinkage in As2O3-induced apoptosis. However, none of these ions contributed significantly to anti-Fas induced depolarisation. This indicates the existence of different mechanisms for apoptotic plasma membrane depolarisation within one cell type.
Florian Nolte; Oliver Friedrich; Markus Rojewski; Rainer H A Fink; Hubert Schrezenmeier; Sixten Körper
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  578     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-02-15     Revised Date:  2005-09-01    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  85-9     Citation Subset:  IM    
Department of Transfusion Medicine, University of Ulm, Institute for Clinical Transfusion Medicine and Immunogenetics, Ulm, Germany.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Antibodies, Monoclonal / metabolism
Antigens, CD95 / immunology,  metabolism*
Antineoplastic Agents / pharmacology*
Apoptosis / physiology*
Arsenicals / pharmacology*
Cell Membrane / metabolism*
Chloride Channels / metabolism
Chlorides / metabolism
Membrane Potentials / drug effects,  physiology*
Myeloid Cells* / cytology,  drug effects,  physiology
Nitrobenzoates / pharmacology
Oxides / pharmacology*
Sodium / metabolism
U937 Cells
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD95; 0/Antineoplastic Agents; 0/Arsenicals; 0/Chloride Channels; 0/Chlorides; 0/Nitrobenzoates; 0/Oxides; 0/anti-Fas monoclonal antibody; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 1327-53-3/arsenic trioxide; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 7440-23-5/Sodium
Erratum In:
FEBS Lett. 2005 Jul 4;579(17):3866

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