Document Detail


Depletion of phagocytes in the reticuloendothelial system causes increased inflammation and mortality in rabbits with Pseudomonas aeruginosa pneumonia.
MedLine Citation:
PMID:  19028978     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phagocytes of the reticuloendothelial system are important in clearing systemic infection; however, the role of the reticuloendothelial system in the response to localized infection is not well-documented. The major goals of this study were to investigate the roles of phagocytes in the reticuloendothelial system in terms of bacterial clearance and inflammatory modulation in sepsis caused by Pseudomonas pneumonia. Macrophages in liver and spleen were depleted by administering liposome encapsulated dichloromethylene diphosphonate (clodronate) intravenously 36 h before the instillation of Pseudomonas aeruginosa into the lungs of anesthetized rabbits. Blood samples were analyzed for bacteria and cytokine concentrations. Lung injury was assessed by the bidirectional flux of albumin and by wet-to-dry weight ratios. Blood pressure and cardiac outputs decreased more rapidly and bacteremia occurred earlier in the clodronate-treated rabbits compared with the nondepleted rabbits. Plasma TNF-alpha (1.08 +/- 0.54 vs. 0.08 +/- 0.02 ng/ml) and IL-8 (6.8 +/- 1.5 vs. 0.0 +/- 0.0 ng/ml) were higher in the depleted rabbits. The concentration of IL-10 in liver of the macrophage-depleted rabbits was significantly lower than in normal rabbits at 5 h. Treatment of macrophage-depleted rabbits with intravenous IL-10 reduced plasma proinflammatory cytokine concentrations and reduced the decline in blood pressure and cardiac output. These results show that macrophages in the reticuloendothelial system have critical roles in controlling systemic bacteremia and reducing systemic inflammation, thereby limiting the systemic effects of a severe pulmonary bacterial infection.
Authors:
Kiyoyasu Kurahashi; Teiji Sawa; Maria Ota; Osamu Kajikawa; Keelung Hong; Thomas R Martin; Jeanine P Wiener-Kronish
Related Documents :
3308778 - Near drowning: consensus and controversies in pulmonary and cerebral resuscitation.
12904188 - A lung recruitment maneuver immediately before rescue surfactant therapy does not affec...
16763218 - Lung recruitment using oxygenation during open lung high-frequency ventilation in prete...
7661378 - Noncardiac pulmonary edema, newer environmental aspects. an update.
18324738 - Pressure and temperature dependence of the chlorine nqr in caesium and sodium chlorates.
590338 - Involvement of central gaba receptors in the regulation of blood pressure and heart rat...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-11-21
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  296     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-27     Completed Date:  2009-03-10     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L198-209     Citation Subset:  IM    
Affiliation:
Dept. of Anesthesiology and Critical Care Medicine, Yokohama City Univ. Graduate School of Medicine, Kanazawa-ku, Yokohama, 236-0004, Japan. kiyok@med.yokohama-cu.ac.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacteremia / etiology,  mortality
Blood Pressure
Clodronic Acid / administration & dosage
Cytokines / metabolism
Enzyme-Linked Immunosorbent Assay
Liposomes
Liver / cytology,  immunology,  microbiology
Lung Injury / etiology,  pathology
Macrophages / physiology
Male
Mononuclear Phagocyte System / metabolism*
Phagocytes / physiology*
Pneumonia, Bacterial / etiology*,  mortality*
Pseudomonas Infections / etiology*,  mortality*
Pseudomonas aeruginosa / pathogenicity
Rabbits
Spleen / cytology,  immunology,  microbiology
Survival Rate
Grant Support
ID/Acronym/Agency:
AI-29103/AI/NIAID NIH HHS; GM-37696/GM/NIGMS NIH HHS; HL-073996/HL/NHLBI NIH HHS; HL-30542/HL/NHLBI NIH HHS; HL-49810/HL/NHLBI NIH HHS; HL-55980/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Liposomes; 10596-23-3/Clodronic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Type 5 phosphodiesterase expression is a critical determinant of the endothelial cell angiogenic phe...
Next Document:  New asthma biomarkers: lessons from murine models of acute and chronic asthma.