| Depletion of mboa-7, an enzyme that incorporates polyunsaturated fatty acids into phosphatidylinositol (PI), impairs PI 3-phosphate signaling in Caenorhabditis elegans. | |
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MedLine Citation:
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PMID: 22862955 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Phosphatidylinositol (PI) is a constituent of biomembranes and a precursor of all phosphoinositides (PIPs). A prominent characteristic of PI is that its sn-2 position is highly enriched in polyunsaturated fatty acids (PUFAs), such as arachidonic acid or eicosapentaenoic acid. However, the biological significance of PUFA-containing PI remains unknown. We previously identified Caenorhabditis elegans (C. elegans) mboa-7 as an acyltransferase that incorporates PUFAs into the sn-2 position of PI. In this study, we performed an RNAi enhancer screen against PI kinases and phosphatases using mboa-7 mutants that have a reduced PUFA content in PI. Among the genes tested, knockdown of vps-34, a catalytic subunit of class III PI 3-kinase that produces PI 3-phosphate (PI3P) from PI, caused severe growth defects in mboa-7 mutants. In both vps-34 RNAi-treated wild-type worms and mboa-7 mutants, the size of PI3P-positive early endosomes was significantly decreased. We also performed an RNAi enhancer screen against PI3P-related genes and found that, like knockdown of vps-34, knockdown of autophagy-related genes caused severe growth defects in mboa-7 mutants. Finally, we showed that autophagic clearance of protein aggregates is impaired in mboa-7 mutants. Taken together, these results suggest that the PUFA chain in PI has a role in some PI3P signaling. |
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Authors:
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Hyeon-Cheol Lee; Takuya Kubo; Nozomu Kono; Eriko Kage-Nakadai; Keiko Gengyo-Ando; Shohei Mitani; Takao Inoue; Hiroyuki Arai |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-1 |
Journal Detail:
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Title: Genes to cells : devoted to molecular & cellular mechanisms Volume: - ISSN: 1365-2443 ISO Abbreviation: Genes Cells Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607379 Medline TA: Genes Cells Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 The Authors Journal compilation © 2012 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd. |
Affiliation:
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Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo, 113-0033, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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