| Depletion of hCINAP by RNA interference causes defects in Cajal body formation, histone transcription, and cell viability. | |
| | |
MedLine Citation:
|
PMID: 20186459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
hCINAP is a highly conserved and ubiquitously expressed protein in eukaryotic organisms and its overexpression decreases the average number of Cajal bodies (CBs) with diverse nuclear functions. Here, we report that hCINAP is associated with important components of CBs. Depletion of hCINAP by RNA interference causes defects in CB formation and disrupts subcellular localizations of its components including coilin, survival motor neurons protein, spliceosomal small nuclear ribonucleoproteins, and nuclear protein ataxia-telangiectasia. Moreover, knockdown of hCINAP expression results in marked reduction of histone transcription, lower levels of U small nuclear RNAs (U1, U2, U4, and U5), and a loss of cell viability. Detection of increased caspase-3 activities in hCINAP-depleted cells indicate that apoptosis is one of the reasons for the loss of viability. Altogether, these data suggest that hCINAP is essential for the formation of canonical CBs, histone transcription, and cell viability. |
| | |
Authors:
|
Jinfang Zhang; Feiyun Zhang; Xiaofeng Zheng |
Related Documents
:
|
20979029 - Expression of class i histone deacetylases during chick and mouse development. 17708529 - Interplay between chromatin remodelers and protein arginine methyltransferases. 20110259 - Involvement of histone deacetylation in morc2-mediated down-regulation of carbonic anhy... 17923479 - Inhibition of histone deacetylase activity promotes invasion of human cancer cells thro... 18204789 - Aging and anti-aging: unexpected side effects of everyday medication through sirtuin1 m... 2905689 - Polyadenylation of histone h3 and h4 mrnas in dicotyledonous plants. 16393119 - Eupatilin inhibits proliferation of ras-transformed human breast epithelial (mcf-10a-ra... 10951979 - Comparison of northern blot hybridization and a reverse transcriptase-polymerase chain ... 7217199 - Regulation of the synthesis of lactate dehydrogenase-x during spermatogenesis in the mo... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-26 |
Journal Detail:
|
Title: Cellular and molecular life sciences : CMLS Volume: 67 ISSN: 1420-9071 ISO Abbreviation: Cell. Mol. Life Sci. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-11 Completed Date: 2010-05-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9705402 Medline TA: Cell Mol Life Sci Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 1907-18 Citation Subset: IM |
Affiliation:
|
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Base Sequence Cell Cycle Cell Cycle Proteins / metabolism Cell Line Cell Survival Chromosomal Proteins, Non-Histone / metabolism Coiled Bodies / metabolism* DNA Primers / genetics Hela Cells Histones / genetics* Humans Nuclear Proteins / antagonists & inhibitors*, genetics*, metabolism, physiology RNA Interference RNA, Small Interfering / genetics RNA, Small Nuclear / metabolism SMN Complex Proteins / metabolism Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/DNA Primers; 0/Histones; 0/NPAT protein, human; 0/Nuclear Proteins; 0/RNA, Small Interfering; 0/RNA, Small Nuclear; 0/SE20-4 protein, human; 0/SMN Complex Proteins; 0/fibrillarin; 136882-81-0/p80-coilin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A (629)RKLKK (633) motif in the hinge region controls the androgen receptor at multiple levels.
Next Document: A FQHPSFI peptide selectively binds to LPS-activated alveolar macrophages and inhibits LPS-induced M...