Document Detail


Depletion of endonuclease G selectively kills polyploid cells.
MedLine Citation:
PMID:  17471024     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endonuclease G is a mitochondrio-nuclear located nuclease with dual-vital and lethal-functions. Besides its role in apoptosis execution, we have recently shown that depletion of endonuclease G leads to necrotic cell death in yeast. Here, we present further mechanistic elucidation of endonuclease G's vital functions. The deletion of the yeast Endonuclease G gene causes the complete elimination of tetraploid cells during exponential growth. Consistently, conditional knockdown of mammalian endonuclease G selectively kills tetraploid but not diploid clones of the human HCT116 colon carcinoma cell line. We conclude that endonuclease G is important for the viability of polyploid mammalian and yeast cells.
Authors:
Sabrina Büttner; Didac Carmona-Gutierrez; Ilio Vitale; Maria Castedo; Doris Ruli; Tobias Eisenberg; Guido Kroemer; Frank Madeo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-27
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  6     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-04     Completed Date:  2007-08-07     Revised Date:  2007-10-04    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1072-6     Citation Subset:  IM    
Affiliation:
Institute of Molecular Biosciences, University of Graz, Graz, Austria.
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MeSH Terms
Descriptor/Qualifier:
Cell Death / physiology*
DNA, Fungal / metabolism
Endodeoxyribonucleases / genetics,  metabolism*
Gene Deletion
Genes, Fungal / physiology*
HCT116 Cells
Humans
Polyploidy*
RNA, Small Interfering / metabolism
Saccharomyces cerevisiae / enzymology,  metabolism
Transfection
Chemical
Reg. No./Substance:
0/DNA, Fungal; 0/RNA, Small Interfering; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/endonuclease G

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