| Depletion of endonuclease G selectively kills polyploid cells. | |
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MedLine Citation:
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PMID: 17471024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endonuclease G is a mitochondrio-nuclear located nuclease with dual-vital and lethal-functions. Besides its role in apoptosis execution, we have recently shown that depletion of endonuclease G leads to necrotic cell death in yeast. Here, we present further mechanistic elucidation of endonuclease G's vital functions. The deletion of the yeast Endonuclease G gene causes the complete elimination of tetraploid cells during exponential growth. Consistently, conditional knockdown of mammalian endonuclease G selectively kills tetraploid but not diploid clones of the human HCT116 colon carcinoma cell line. We conclude that endonuclease G is important for the viability of polyploid mammalian and yeast cells. |
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Authors:
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Sabrina Büttner; Didac Carmona-Gutierrez; Ilio Vitale; Maria Castedo; Doris Ruli; Tobias Eisenberg; Guido Kroemer; Frank Madeo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-05-27 |
Journal Detail:
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Title: Cell cycle (Georgetown, Tex.) Volume: 6 ISSN: 1551-4005 ISO Abbreviation: Cell Cycle Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-05-04 Completed Date: 2007-08-07 Revised Date: 2007-10-04 |
Medline Journal Info:
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Nlm Unique ID: 101137841 Medline TA: Cell Cycle Country: United States |
Other Details:
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Languages: eng Pagination: 1072-6 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biosciences, University of Graz, Graz, Austria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Death
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physiology* DNA, Fungal / metabolism Endodeoxyribonucleases / genetics, metabolism* Gene Deletion Genes, Fungal / physiology* HCT116 Cells Humans Polyploidy* RNA, Small Interfering / metabolism Saccharomyces cerevisiae / enzymology, metabolism Transfection |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 0/RNA, Small Interfering; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.21.-/endonuclease G |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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