Document Detail


Depletion of L-3,5,3'-triiodothyronine and L-thyroxine in euthyroid calf serum for use in cell culture studies of the action of thyroid hormone.
MedLine Citation:
PMID:  446419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GH1 cells are a clonal strain of rat pituitary tumor cells which synthesize GH and PRL. We have previously demonstrated that these cells respond to physiological concentrations of L-T3 and L-T4 when cultured with medium supplemented with thyroidectomized calf serum to achieve a thyroid hormone-depleted state under cell culture conditions. In this study, we describe a method to deplete euthyroid calf serum of L-T3 and L-T4 using an anion exchange resin. We demonstrate that the procedure only minimally alters the low molecular weight anion components of the serum and does not change the total protein content or the electrophoretic pattern of serum proteins. Moreover, we show that euthyroid calf serum depleted of L-T3 and L-T4 by this procedure yields serum which, when used as a medium supplement, results in biological responses identical to those obtained with media supplemented with thyroidectomized calf serum. In addition, resin treatment does not alter the growth-promoting properties of the serum if the thyroid hormone concentration is restored. This procedure should be useful in preparing thyroid hormone-depleted serum for cell culture studies in situations where thyroidectomy is not feasible or would require surgical procedures on a large number of small animals.
Authors:
H H Samuels; F Stanley; J Casanova
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  105     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-09-01     Completed Date:  1979-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  80-5     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood*
Cattle
Cell Division
Cell Line
Chromatography, Ion Exchange
Culture Media*
Growth Hormone / biosynthesis
Hydrocortisone / pharmacology
Methods
Rats
Thyroxine / blood*
Triiodothyronine / blood*,  pharmacology
Chemical
Reg. No./Substance:
0/Culture Media; 50-23-7/Hydrocortisone; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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