| Depletion of 4-hydroxynonenal in hGSTA4-transfected HLE B-3 cells results in profound changes in gene expression. | |
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MedLine Citation:
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PMID: 16005854 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we have shown that overexpression of 4-hydroxy-2-nonenal (HNE)-detoxifying enzyme glutathione S-transferase A4-4 (hGSTA4-4) in human lens epithelial cells (HLE B-3) leads to pro-carcinogenic phenotypic transformation of these cells [R. Sharma, et al. Eur. J. Biochem. 271 (2004) 1960-1701]. We now demonstrate that hGSTA4-transfection also causes a profound change in the expression of genes involved in cell adhesion, cell cycle control, proliferation, cell growth, and apoptosis, which is consistent with phenotypic changes of the transformed cells. The expression of p53, p21, p16, fibronectin 1, laminin gamma1, connexin 43, Fas, integrin alpha6, TGFalpha, and c-jun was down-regulated, while the expression of protein kinase C beta II (PKCbetaII), c-myc, cyclin-dependent kinase 2 (CDK2), and TGFbeta was up-regulated in transfected cells. These results demonstrate that HNE serves as a crucial signaling molecule and, by modulating the expression of genes, can influence cellular functions. |
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Authors:
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Brad Patrick; Jie Li; Prince V S Jeyabal; Prasada M R V Reddy; Yusong Yang; Rajendra Sharma; Mala Sinha; Bruce Luxon; Piotr Zimniak; Sanjay Awasthi; Yogesh C Awasthi |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 334 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-07-22 Completed Date: 2005-09-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 425-32 Citation Subset: IM |
Affiliation:
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Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aldehydes
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metabolism* Cell Line Cell Transformation, Neoplastic / metabolism* Epithelium, Corneal / metabolism* Gene Expression Profiling Gene Expression Regulation / physiology* Glutathione Transferase / genetics, metabolism* Humans Recombinant Proteins / metabolism Signal Transduction / physiology* Transfection / methods |
| Grant Support | |
ID/Acronym/Agency:
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CA77495/CA/NCI NIH HHS; ES 007254/ES/NIEHS NIH HHS; ES 07804/ES/NIEHS NIH HHS; ES021171/ES/NIEHS NIH HHS; ES06676/ES/NIEHS NIH HHS; EY 04396/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aldehydes; 0/Recombinant Proteins; 29343-52-0/4-hydroxy-2-nonenal; EC 2.5.1.18/Glutathione Transferase; EC 4.4.1.20/leukotriene-C4 synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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