| Depicting the spatial distribution of proteins in human tumor tissue combining SELDI and MALDI imaging and immunohistochemistry. | |
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MedLine Citation:
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PMID: 20644210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carcinoma tissue consists of not only tumor cells but also fibroblasts, endothelial cells or vascular structures, and inflammatory cells forming the supportive tumor stroma. Therefore, the spatial distribution of proteins that promote growth and proliferation in these complex functional units is of high interest. Matrix-assisted laser desorption/ionization imaging mass spectrometry is a newly developed technique that generates spatially resolved profiles of protein signals directly from thin tissue sections. Surface-enhanced laser desorption/ionization mass spectrometry (MS)combined with tissue microdissection allows analysis of defined parts of the tissue with a higher sensitivity and a broader mass range. Nevertheless, both MS-based techniques have a limited spatial resolution. IHC is a technique that allows a resolution down to the subcellular level. However, the detection and measurement of a specific protein expression level is possible only by semiquantitative methods. Moreover, prior knowledge about the identity of the proteins of interest is necessary. In this study, we combined all three techniques to gain highest spatial resolution, sensitivity, and quantitative information. We used frozen tissue from head and neck tumors and chose two exemplary proteins (HNP1-3 and S100A8) to highlight the advantages and disadvantages of each technique. It could be shown that the combination of these three techniques results in congruent but also synergetic data. |
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Authors:
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Liane Wehder; Günther Ernst; Anna C Crecelius; Orlando Guntinas-Lichius; Christian Melle; Ulrich S Schubert; Ferdinand von Eggeling |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-19 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 58 ISSN: 1551-5044 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2010-10-06 Revised Date: 2012-04-27 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 929-37 Citation Subset: IM |
Affiliation:
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Core Unit Chip Application, Institut für Humangenetik, University Hospital Jena, Jena, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calgranulin A
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metabolism* Head and Neck Neoplasms / metabolism* Humans Immunohistochemistry Microdissection Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods alpha-Defensins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Calgranulin A; 0/alpha-Defensins; 0/human neutrophil peptide 1; 0/human neutrophil peptide 2; 0/human neutrophil peptide 3 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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