Document Detail


Dephosphorylation of the retinoblastoma protein during differentiation of HL60 cells.
MedLine Citation:
PMID:  1338571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunoprecipitated retinoblastoma protein from HL60 cells migrated as a series of bands during electrophoresis. The heterogeneity appeared to be generated by phosphorylation of the retinoblastoma protein. Treatment of the cells with the phorbol ester, tetradecanoyl phorbol acetate (TPA), resulted in both a loss of the heterogeneity of the pRB species and a significant decrease in the level of pRB phosphorylation. These changes accompanied differentiation of the HL60 cells into macrophages. Treatment of the cells with dibutyryl cAMP also resulted in dephosphorylation of pRB as well as cell cycle arrest, although no recognizable differentiation occurred. These results are consistent with a model in which TPA and dibutyryl cAMP dependent pathways can activate pRB by altering its phosphorylation.
Authors:
P Whyte; R N Eisenman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry and cell biology = Biochimie et biologie cellulaire     Volume:  70     ISSN:  0829-8211     ISO Abbreviation:  Biochem. Cell Biol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-05-20     Completed Date:  1993-05-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8606068     Medline TA:  Biochem Cell Biol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  1380-4     Citation Subset:  IM    
Affiliation:
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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MeSH Terms
Descriptor/Qualifier:
Bucladesine / pharmacology
Cell Differentiation
Cyclic AMP / pharmacology
Humans
Immunosorbent Techniques
Leukemia, Promyelocytic, Acute / metabolism*,  pathology
Macrophages / pathology
Phosphorylation
Protein Kinase C / metabolism
Retinoblastoma Protein / metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
R01 CA20525/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Retinoblastoma Protein; 16561-29-8/Tetradecanoylphorbol Acetate; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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