| Dephosphorylation of the retinoblastoma protein during differentiation of HL60 cells. | |
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MedLine Citation:
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PMID: 1338571 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunoprecipitated retinoblastoma protein from HL60 cells migrated as a series of bands during electrophoresis. The heterogeneity appeared to be generated by phosphorylation of the retinoblastoma protein. Treatment of the cells with the phorbol ester, tetradecanoyl phorbol acetate (TPA), resulted in both a loss of the heterogeneity of the pRB species and a significant decrease in the level of pRB phosphorylation. These changes accompanied differentiation of the HL60 cells into macrophages. Treatment of the cells with dibutyryl cAMP also resulted in dephosphorylation of pRB as well as cell cycle arrest, although no recognizable differentiation occurred. These results are consistent with a model in which TPA and dibutyryl cAMP dependent pathways can activate pRB by altering its phosphorylation. |
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Authors:
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P Whyte; R N Eisenman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 70 ISSN: 0829-8211 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 1992 Dec |
Date Detail:
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Created Date: 1993-05-20 Completed Date: 1993-05-20 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: CANADA |
Other Details:
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Languages: eng Pagination: 1380-4 Citation Subset: IM |
Affiliation:
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Fred Hutchinson Cancer Research Center, Seattle, WA 98104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bucladesine
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pharmacology Cell Differentiation Cyclic AMP / pharmacology Humans Immunosorbent Techniques Leukemia, Promyelocytic, Acute / metabolism*, pathology Macrophages / pathology Phosphorylation Protein Kinase C / metabolism Retinoblastoma Protein / metabolism* Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA20525/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Retinoblastoma Protein; 16561-29-8/Tetradecanoylphorbol Acetate; 362-74-3/Bucladesine; 60-92-4/Cyclic AMP; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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