Document Detail


Dephosphorylation of circulating human osteopontin correlates with severe valvular calcification in patients with calcific aortic valve disease.
MedLine Citation:
PMID:  22191734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CONTEXT: Calcific Aortic Valve Disease (CAVD) is an active pathological process leading to biomineralization of the aortic cusps. We characterized circulating and tissue Osteopontin (OPN) as a biomarker for CAVD.
OBJECTIVES: Here we investigate the post-translational modifications of circulating OPN and correlate the phosphorylation status with the ability to prevent calcification.
METHODS: Circulating OPN levels were estimated in CAVD patients (n = 51) and controls (n = 56). In a subgroup of 27 subjects, OPN was purified and the phosphorylation status analyzed.
RESULTS: Plasma OPN levels were significantly elevated in CAVD patients as compared to the controls and correlates with the aortic valve calcium score. Our study demonstrates that phospho-threonine levels of OPN purified from controls were higher when compared to CAVD subjects, whereas phospho-serine and phospho-tyrosine levels were comparable between the two groups.
CONCLUSION: The dephosphorylation of circulating OPN correlates with severe valvular calcification in patients with CAVD.
Authors:
Rachana Sainger; Juan B Grau; Paolo Poggio; Emanuela Branchetti; Joseph E Bavaria; Joseph H Gorman; Robert C Gorman; Giovanni Ferrari
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-23
Journal Detail:
Title:  Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals     Volume:  17     ISSN:  1366-5804     ISO Abbreviation:  Biomarkers     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-01     Completed Date:  2012-07-23     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  9606000     Medline TA:  Biomarkers     Country:  England    
Other Details:
Languages:  eng     Pagination:  111-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Aortic Valve / metabolism*,  pathology
Aortic Valve Stenosis / blood,  diagnosis*,  pathology
Biological Markers / blood*
Calcinosis*
Calcium / blood
Case-Control Studies
Female
Humans
Male
Osteopontin / blood*
Phosphorylation
Phosphothreonine / blood
Protein Processing, Post-Translational*
Severity of Illness Index
Grant Support
ID/Acronym/Agency:
R01 HL103723/HL/NHLBI NIH HHS; RC1 HL100035/HL/NHLBI NIH HHS; RC1 HL100035-02/HL/NHLBI NIH HHS; RC1HL100035/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 106441-73-0/Osteopontin; 1114-81-4/Phosphothreonine; SY7Q814VUP/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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