Document Detail


Dependence of myocardial hypothermia tolerance on sources of activator calcium.
MedLine Citation:
PMID:  9367607     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the relationship between cardiac hypothermia tolerance and the sources of activator calcium, we selectively modified either the sarcolemmal calcium permeability by nifedipine or the sarcoplasmic reticulum function by caffeine in papillary muscles from both the rat, as a cold sensitive model, and the ground squirrel, Citellus dauricus, a deep hibernator. Both force-interval relationship and cooling performance were investigated. At 25 degrees C, the slope of the force-interval curve of the ground squirrel was nearly double that of the rat. At shorter test intervals 0.5 muM nifedipine moved the curve down with little effect at longer intervals, and the curve slope increased. Caffeine (1 mM) decreased the peak force and eliminated its dependence upon test interval. When the temperature was lowered, rat preparations showed a marked increase of resting tension and aftercontraction between 7 and 12 degrees C and became inexcitable. In contrast, they maintained contractility down to a few degrees above 0 degrees C without aftercontraction and increased resting tension in the ground squirrel. In the rat nifedipine shortened the contractions, prevented the increase of resting tension, and minimized aftercontractions, with little improvement of contractility. Caffeine prolonged the contractions, caused a striking increase of resting tension and aftercontractions, and finally disabled the contractility at about 5-10 degrees C, even in the ground squirrel. We conclude that depressed calcium influx helps to prevent hypothermic calcium overload of the cardiac cells. Good function of the sarcoplasmic reticulum is essential for tolerance of hypothermia by cardiac cells. A suggestion that may improve the hypothermic tolerance of the myocardium from nonhibernators is postulated.
Authors:
S Q Wang; Y H Huang; K S Liu; Z Q Zhou
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cryobiology     Volume:  35     ISSN:  0011-2240     ISO Abbreviation:  Cryobiology     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-06     Completed Date:  1998-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  193-200     Citation Subset:  IM; S    
Copyright Information:
Copyright 1997 Academic Press.
Affiliation:
College of Life Sciences, Peking University, Beijing, 100871, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological
Animals
Caffeine / pharmacology
Calcium / metabolism*
Calcium Channel Blockers / pharmacology
Female
Hibernation / physiology
Hypothermia / metabolism*,  physiopathology
Hypothermia, Induced
Male
Myocardial Contraction / drug effects
Myocardium / metabolism*
Nifedipine / pharmacology
Rats
Sciuridae
Species Specificity
Chemical
Reg. No./Substance:
0/Calcium Channel Blockers; 21829-25-4/Nifedipine; 58-08-2/Caffeine; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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