Document Detail


Dependence of induced transmembrane potential on cell density, arrangement, and cell position inside a cell system.
MedLine Citation:
PMID:  12046706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A nonuniform transmembrane potential (TMP) is induced on a cell membrane exposed to external electric field. If the induced TMP is above the threshold value, cell membrane becomes permeabilized in a reversible process called electropermeabilization. Studying electric potential distribution on the cell membrane gives us an insight into the effects of the electric field on cells and tissues. Since cells are always surrounded by other cells, we studied how their interactions influence the induced TMP. In the first part of our study, we studied dependence of potential distribution on cell arrangement and density in infinite cell suspensions where cells were organized into simple-cubic, body-centered cubic, and face-centered cubic lattice. In the second part of the study, we examined how induced TMP on a cell membrane is dependent on its position inside a three-dimensional cell cluster. Finally, the results for cells inside the cluster were compared to those in infinite lattice. We used numerical analysis for the study, specifically the finite-element method (FEM). The results for infinite cell suspensions show that the induced TMP depends on both: cell volume fraction and cell arrangement. We established from the results for finite volume cell clusters and layers, that there is no radial dependence of induced TMP for cells inside the cluster.
Authors:
Mojca Pavlin; Natasa Pavselj; Damijan Miklavcic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  IEEE transactions on bio-medical engineering     Volume:  49     ISSN:  0018-9294     ISO Abbreviation:  IEEE Trans Biomed Eng     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-05     Completed Date:  2002-07-19     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0012737     Medline TA:  IEEE Trans Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  605-12     Citation Subset:  IM    
Affiliation:
University of Ljubljana, Faculty of Electrical Engineering, Slovenia.
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MeSH Terms
Descriptor/Qualifier:
Cell Count
Cell Physiological Phenomena
Cell Size
Computer Simulation
Electromagnetic Fields
Extracellular Space / physiology*
Finite Element Analysis
Membrane Potentials / physiology*,  radiation effects*
Models, Biological*
Reproducibility of Results
Sensitivity and Specificity
Suspensions
Chemical
Reg. No./Substance:
0/Suspensions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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