| Dependence of acetylcholine and ADP dilation of pial arterioles on heme oxygenase after transfusion of cell-free polymeric hemoglobin. | |
| | |
MedLine Citation:
|
PMID: 16214847 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Polymers of cell-free hemoglobin have been designed for clinical use as oxygen carriers, but limited information is available regarding their effects on vascular regulation. We tested the hypothesis that the contribution of heme oxygenase (HO) to acetylcholine-evoked dilation of pial arterioles is upregulated 2 days after polymeric hemoglobin transfusion. Dilator responses to acetylcholine measured by intravital microscopy in anesthetized cats were blocked by superfusion of the HO inhibitor tin protoporphyrin-IX (SnPPIX) in a group that had undergone exchange transfusion with hemoglobin 2 days earlier but not in surgical sham and albumin-transfused groups. However, immunoblots from cortical brain homogenates did not reveal changes in expression of the inducible isoform HO1 or the constitutive isoform HO2 in the hemoglobin-transfused group. To test whether the inhibitory effect of SnPPIX was present acutely after hemoglobin transfusion, responses were measured within an hour of completion of the exchange transfusion. In control and albumin-transfused groups, acetylcholine responses were unaffected by SnPPIX but were blocked by addition of the nitric oxide synthase inhibitor N(omega)-nitro-l-arginine (l-NNA) to the superfusate. In hemoglobin-transfused groups, the acetylcholine response was blocked by either SnPPIX or l-NNA alone. The effect of another HO inhibitor, chromium mesoporphyrin (CrMP), was tested on ADP, another endothelial-dependent dilator, in anesthetized rats. Pial arteriolar dilation to ADP was unaffected by CrMP in controls but was attenuated 62% by CrMP in rats transfused with hemoglobin. It is concluded that 1) polymeric hemoglobin transfusion acutely upregulates the contribution of HO to acetylcholine-induced dilation of pial arterioles in cats, 2) this upregulation persists 2 days after transfusion when 95% of the hemoglobin is cleared from the circulation, and 3) this acute upregulation of HO signaling is ubiquitous in that similar effects were observed with a different endothelial-dependent agonist (i.e., ADP) in a another species (rat). |
| | |
Authors:
|
Annette Rebel; Suyi Cao; Herman Kwansa; Sylvain Doré; Enrico Bucci; Raymond C Koehler |
Related Documents
:
|
19869747 - Studies in the blood cytology of the rabbit : ii. consecutive erythrocyte and hemoglobi... 18649167 - Effects of pegylated hamster red blood cells on microcirculation. 10619927 - Peg-hemoglobin as a resuscitation solution in the treatment of hypovolemic shock in the... 1611887 - A flow procedure to determine oxygen binding isotherms for low affinity and easily oxid... 15691037 - Use of a time-resolved immunofluorometric assay for determination of canine c-reactive ... 7124307 - Nitroprusside-hypotension: cerebral blood flow and cerebral oxygen consumption in neuro... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2005-10-07 |
Journal Detail:
|
Title: American journal of physiology. Heart and circulatory physiology Volume: 290 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2006 Mar |
Date Detail:
|
Created Date: 2006-02-09 Completed Date: 2006-04-13 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: H1027-37 Citation Subset: IM |
Affiliation:
|
Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University, Baltimore, Maryland 21287, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylcholine
/
metabolism* Adenosine Diphosphate / metabolism* Animals Arterioles / drug effects, metabolism* Blood Substitutes / administration & dosage* Blood Transfusion Cats Heme Oxygenase (Decyclizing) / metabolism* Hemoglobins / administration & dosage* Male Pia Mater / blood supply*, drug effects, metabolism* Vasodilation / drug effects, physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
NS-38384/NS/NINDS NIH HHS; R01 NS038684-07/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Blood Substitutes; 0/Hemoglobins; 51-84-3/Acetylcholine; 58-64-0/Adenosine Diphosphate; EC 1.14.99.3/Heme Oxygenase (Decyclizing) |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Functional and structural remodeling of the myocardial microvasculature in early experimental hypert...
Next Document: Optical mapping of late myocardial infarction in rats.